河南省烟草疫霉遗传多样性与遗传结构的RAPD分析  

Genetic Diversity and Genetic Structure Analysis of Phytophthora nicotianae in Henan by RAPD

作　　者：付静 崔林开[2] 付伟 乔莉娟 FU Jing;CUI Linkai;FU Wei;QIAO Lijuan(School of Life Science and Engineering,Handan University,Handan 056005,China;Forestry College,Henan University of Science and Technology,Luoyang 471023,China)

机构地区：[1]邯郸学院生命科学与工程学院,河北邯郸056005 [2]河南科技大学林学院,河南洛阳471023

出　　处：《华北农学报》2020年第S01期342-346,共5页Acta Agriculturae Boreali-Sinica

基　　金：国家自然科学基金项目(U1404318);河北省教育厅科技指导项目(Z2019005);邯郸学院2017年度校级资助项目(2017102)。

摘　　要：为了明确河南省烟草疫霉群体的遗传多样性与遗传结构,利用RAPD分子标记对河南省的34个烟草疫霉菌株进行分析。结果表明,6条RAPD引物共扩增出30个条带,多态性条带比率为90%;Nei’s基因多样性指数为0.2710,Shannon信息指数为0.4028;菌株间的遗传相似系数在0.59~1.00,相似系数0.80水平下UPGMA聚类可将34个菌株划分为6个类群,类群Ⅰ包括26个菌株,为优势类群;主成分分析结果与UPGMA聚类结果相一致;遗传结构分析推测河南烟草疫霉群体起源于2个祖先亚群,这2个亚群在群体中所占比例分别为72.73%,27.27%,亚群Ⅰ为优势亚群;单个菌株遗传构成分析显示55.88%的菌株遗传组分非常单一。因此,河南省烟草疫霉群体的遗传多样性不丰富,遗传结构也较为简单。To determine the genetic diversity and genetic structure of Phytophthora nicotianae from Henan Province,34 P.nicotianae isolates were analyzed by randomly amplified polymorphic DNA(RAPD)marker.The results showed that 30 bands were amplified using 6 RAPD primers and 90%of them were polymorphic.Nei′s gene diversity index was 0.2710,and Shannon′s information index was 0.4028.The genetic similarity coefficient of 34 isolates ranged from 0.59 to 1.00.Based on UPGMA dendrogram,34 isolates were divided into 6 groups with 0.80 genetic similarity.GroupⅠwas the dominant group including 26 isolates.The result of principle coordinate analysis was basically similar to that of UPGMA dendrogram.The result of genetic structure analysis revealed that P.nicotianae population in Henan originated from 2 ancestral subpopulations.The subpopulationⅠand subpopulationⅡaccounted for 72.73%,27.27%,respectively.The subpopulationⅠwas the dominant subpopulation.Genetic component of 55.88%of isolates almost consisted of only one subpopulation.Therefore,the genetic diversity of P.nicotianae from Henan was low and the genetic structure of them was also simple.

关 键 词：烟草疫霉 随机扩增多态性DNA 遗传多样性 遗传结构 

分 类 号：S432.1[农业科学—植物病理学]