肝细胞核因子1α在慢性乙型肝炎患者肝脏炎症及纤维化发生发展过程中的表达变化及其意义  

Alteration on hepatocyte nuclear factor 1αexpressions and significance in the process of occurrence and development of liver inflammation and fibrosis in patients with chronic hepatitis B

作　　者：邓羊羊 葛善飞[1] 余燕青[2] 赖玲玲[1] 王亮[1] 李文成 徐玲君 傅文山 熊英[1] Deng Yangyang;Ge Shanfei;Yu Yanqing;Lai Lingling;Wang Liang;Li Wencheng;Xu Lingjun;Fu Wenshan;Xiong Ying(Department of Infectious Diseases,the First Affiliated Hospital of Nanchang University,Nanchang 330006,China;Department of Pathology,the First Affiliated Hospital of Nanchang University,Nanchang 330006,China;Department of Infection Control,the First Affiliated Hospital of Nanchang University,Nanchang 330006,China)

机构地区：[1]南昌大学第一附属医院感染性疾病科,330006 [2]南昌大学第一附属医院病理科,330006 [3]南昌大学第一附属医院感染控制处,330006

出　　处：《中华肝脏病杂志》2021年第2期121-125,共5页Chinese Journal of Hepatology

基　　金：国家自然科学基金地区基金项目(81660109)。

摘　　要：目的探讨肝细胞核因子(HNF)1α在慢性乙型肝炎患者肝组织中的表达与肝组织炎症和纤维化发生、发展的关系。方法选择2011年至2018年诊治的慢性乙型肝炎患者64例,患者均已在超声引导下行肝穿刺活组织检查术,收集其肝穿刺病理学结果进行炎症分级及纤维化分期;收集穿刺肝组织蜡块进行切片,采用免疫组织化学染色法检测HNF1α在肝组织中的表达情况。双向有序分组资料采用Mantel-Haenszelχ^(2)检验,等级相关性分析采用Spearman等级秩相关性检验。结果慢性乙型肝炎患者肝组织呈现出不同程度的炎症坏死及纤维化,HNF1α表达与肝组织炎症水平分级存在线性关系(χ^(2)MH=40.70,P<0.05),慢性乙型肝炎患者肝组织炎症水平越高,HNF1α在肝组织中的表达越弱,HNF1α表达强度与炎症分级呈负相关(rs=-0.815,P<0.05);HNF1α表达与肝组织纤维化程度分期存在线性关系(χ^(2)MH=31.95,P<0.05),随着肝纤维化程度加重,HNF1α在肝组织中的表达水平逐渐降低,HNF1α表达强度与纤维化分期呈负相关(rs=-0.713,P<0.05)。结论HNF1α与肝组织炎症及纤维化的发生、发展密切相关,有望成为评价慢性乙型肝炎患者肝组织炎症及纤维化水平的敏感指标,其表达下调可能参与了肝脏炎症及肝纤维化发生、发展的过程,有可能成为慢性肝炎治疗的新靶点。Objective To investigate the relationship between the expression of hepatocyte nuclear factor-1α(HNF-1α)and the occurrence and development of liver inflammation and fibrosis in liver tissues of patients with chronic hepatitis B.Methods Sixty-four patients with chronic hepatitis B who were diagnosed and treated in our hospital from 2011 to 2018 were selected.All patients underwent ultrasound-guided aspiration liver biopsy.The pathological results of liver biopsy were collected for inflammation grading and fibrosis staging.The liver puncture biopsies was collected by paraffin sectioning.The expression of HNF1αin the liver tissue was detected by immunohistochemical staining.Mantel-Haenszelχ^(2)test was used for bidirectional ordered grouping data,and Spearman’s rank-correlation test was used for rank correlation analysis.Results There were varying degrees of inflammatory necrosis and fibrosis in the liver tissues of patients with chronic hepatitis B.There was a linear relationship between the expression of HNF1αand the level of inflammation in liver tissues(χ^(2)MH=40.70,P<0.05).The expression of HNF1αin liver tissues of patients with chronic hepatitis B was decreased with the increase of liver inflammation.The expression intensity of HNF1αwas negatively correlated with the inflammation grade(rs=-0.815,P<0.05).There was a linear relationship between the expressions of HNF1αand the degree and stage of liver fibrosis(χ^(2)MH=31.95,P<0.05).The expression level of HNF1αin liver tissue was gradually decreased with the aggravation of liver fibrosis.The expression intensity of HNF1αwas negatively correlated with fibrosis stage(rs=-0.713,P<0.05).Conclusion HNF1αis closely related to the occurrence and development of liver tissue inflammation and fibrosis,and is expected to be a sensitive indicator for evaluating the level of liver tissue inflammation and fibrosis in patients with chronic hepatitis B.In addition,its down-regulation may be involved in the process of occurrence and development of liver inflammat

关 键 词：乙型肝炎 慢性 肝细胞核因子1α 炎症 纤维化 

分 类 号：R512.62[医药卫生—内科学]