Janus激酶/信号转导及转录激活子3通路抑制剂对严重烧伤大鼠骨骼肌功能的影响及其机制  

Effects of Janus kinase/signal transduction and activator of transcription 3 pathway inhibitor in skeletal muscle function in severely burned rats and its mechanism

作　　者：白海良 段红杰[2] 陈晨[3] 刘玲英[2] 吴育寿 韩绍芳 王晓腾[2] Bai Hailiang;Duan Hongjie;Chen Chen;Liu Lingying;Wu Yushou;Han Shaofang;Wang Xiaoteng(The Second Clinical Medical College of Shanxi Medical University,Taiyuan 030001,China;Department of Burns and Plastic Surgery,the Fourth Medical Center of PLA General Hospital,Beijing 100048,China;Department of Burns and Traumatic Surgery,Hainan Hospital of PLA General Hospital,Sanya 572013,China)

机构地区：[1]山西医科大学第二临床医学院,大原030001 [2]解放军总医院第四医学中心烧伤整形科,北京100048 [3]解放军总医院海南医院战创伤救治科,三亚572013

出　　处：《中华烧伤杂志》2021年第3期271-278,共8页Chinese Journal of Burns

基　　金：海南省自然科学基金面上项目(817348);军委后勤保障部重大项目(ALP19J001)。

摘　　要：目的观察严重烧伤后大鼠骨骼肌功能变化,并探讨Janus激酶/信号转导及转录激活子3(JAK/STAT3)通路抑制剂对骨骼肌功能的影响及可能的机制。方法采用实验研究方法。取120只8周龄雄性Wistar大鼠,按随机数字表法分为假伤组、单纯烧伤组和烧伤+JAK/STAT3抑制剂组,每组40只,后2组大鼠于背部、腹部造成50%体表总面积Ⅲ度烫伤,假伤组大鼠致假伤。烧伤+JAK/STAT3抑制剂组大鼠腹腔注射JAK/STAT3抑制剂鲁索替尼。伤后0(即刻)、1、4、7、14 d,每组取8只大鼠,采用多通道电生理仪测量脉冲频率20、40、60、80、100、120、140、160 Hz刺激最佳肌肉长度的趾长伸肌产生的比力,测量脉冲频率50 Hz刺激0、10、20、30、60、120、180、240、300 s的最佳肌肉长度的趾长伸肌疲劳期比力,采用紫外分光光度法检测趾长伸肌羰基化合物含量,采用微量法检测趾长伸肌ATP含量。对数据行重复测量方差分析、析因设计方差分析、Bonferroni法、t检验。结果与假伤组比较,单纯烧伤组大鼠伤后0、1、7 d各脉冲频率,伤后4 d除20 Hz外各脉冲频率,伤后14 d于20、40 Hz脉冲频率刺激后趾长伸肌比力均显著下降(P<0.05或P<0.01)。与单纯烧伤组相比,烧伤+JAK/STAT3抑制剂组大鼠伤后1 d除20 Hz外各脉冲频率,伤后4、7、14 d各脉冲频率刺激后趾长伸肌比力显著升高(P<0.05或P<0.01)。与假伤组比较,单纯烧伤组大鼠除伤后7 d刺激240 s外,伤后各时间点刺激各时间点趾长伸肌疲劳期比力显著下降(P<0.05或P<0.01)。与单纯烧伤组比较,烧伤+JAK/STAT3抑制剂组大鼠伤后1 d除刺激60、300 s外各刺激时间点,伤后4 d除刺激240 s外各刺激时间点,伤后7、14 d所有刺激时间点疲劳期比力显著升高(P<0.05或P<0.01)。单纯烧伤组大鼠伤后0、1、4、7、14 d趾长伸肌羰基化合物含量分别为(0.651±0.155)、(0.739±0.194)、(0.618±0.086)、(0.813±0.162)、(0.615±0.115)nmol/mg,明显高于烧伤+JObjective To observe the functional changes of skeletal muscle in severely burned rats,and to investigate the effects and possible mechanisms of Janus kinase/signal transducer and activator of transcription 3(JAK/STAT3)pathway inhibitor in skeletal muscle function.Methods The experiment research method was applied.One hundred and twenty male Wistar rats of 8-week-old were divided into sham injury group,simple burn group,and burn+JAK/STAT3 inhibitor group according to the random number table,with 40 rats in each group.Rats in simple burn group and burn+JAK/STAT3 inhibitor group were inflicted with 50%total body surface area full-thickness scald on the back and abdomen,and rats in sham injury group were sham injured.Rats in burn+JAK/STAT3 inhibitor group were intraperitoneally injected with JAK/STAT3 inhibitor ruxolitinib.On post injury day(PID)0(immediately),1,4,7,and 14,8 rats in each group were used to measure the specific force generated by extensor digitorum longus in optimal length stimulated with pulse frequency of 20,40,60,80,100,120,140,and 160 Hz using a multichannel electrophysiological instrument,and specific force in fatigue period of extensor digitorum longus in optimal length stimulated with pulse frequency of 50 Hz for 0,10,20,30,60,120,180,240,and 300 s.On PID 0,1,4,7,and 14,carbonyl compound content of extensor digitorum longus was determined by ultraviolet spectrophotometry,and ATP content of extensor digitorum longus was determined by micrometry.Data were statistically analyzed with analysis of variance for repeated measurement,analysis of variance for factorial design,Bonferroni method,and t test.Results Compared with those of sham injury group,specific forces of extensor digitorum longus of rats in simple burn group were significantly decreased after being stimulated with all the pulse frequency on PID 0,1,7,and all the pulse frequency except for 20 Hz on PID 4,and pulse frequency of 20 and 40 Hz on PID 14(P<0.05 or P<0.01).Compared with those of simple burn group,specific forces of extensor d

关 键 词：烧伤 肌 骨骼 Janus激酶/信号转导及转录激活子3 氧化应激 

分 类 号：R619+.3[医药卫生—外科学] R61[医药卫生—临床医学]