相思杂种优势的转录组初步分析  

Preliminary Analysis on Transcriptome of Heterosis in Acacia

作　　者：张如平 卢艳平 曾炳山[1] 黄烈健[1] Zhang Ruping;Lu Yanping;Zeng Bingshan;Huang Liejian(Research Institute of Tropical Forestry,Chinese Academy of Forestry,Guangzhou,510520)

机构地区：[1]中国林业科学研究院热带林业研究所,广州510520

出　　处：《分子植物育种》2021年第5期1516-1521,共6页Molecular Plant Breeding

基　　金：中国林科院科研业务费专项(CAFYBB2017MB009)资助。

摘　　要：马大杂种相思(Acacia mangium×A.auriculiformis)是生产中极具潜力的相思新树种,为了探索相思杂种优势的分子机理,本研究通过高通量转录组测序技术对大叶相思、马占相思及其子代马大杂种相思的幼嫩叶片进行无参转录组测序分析。结果显示大叶相思、马大杂种相思和马占相思质控数据的Clean reads分别为42595190、55373032和46553286条,有效数据占原始Raw reads的比例在99%以上;Q20、Q30分别高于98%和95%。优化组装后总转录本数量为133473,总Unigenes为83117,序列碱基总数为122735856 bp,其中1000 bp以内的共有63377条,占总数的76%以上。3种相思的Unigenes表达量分布十分接近,共同检测到的Unigenes为20379个,对这些Unigenes进行KEGG功能注释,共注释到129个不同的通路中,其中光合作用、植物激素信号转导通路中的相关基因显著富集。对3种相思注释到map00195(光合通路)中的Unigenes进行表达量的差异分析,发现大部分的Unigenes在马大杂种相思中的表达量均高于双亲。这些Unigenes对应的基因主要为影响光合作用过程的关键基因,且马大杂种相思的光合通路中的相关基因表达量均显著高于双亲,由此推测这是导致杂种相思生长优势的主要原因。本研究结果可为今后开展相思杂交育种提供理论依据。Acacia mangium×A.auriculiformis is a new Acacia species with great potential in production.In order to explore the molecular mechanism of Acacia heterosis,the nonparametric transcriptome sequencing analysis of young leaves of Acacia auriculiformis,Acacia mangium and its progeny Acacia mangium×A.auriculiformis was carried out by high throughput transcriptome sequencing technology.The results showed that there were 42595190,55373032 and 46553286 Clean reads for the quality control data of Acacia auriculiformis,Acacia mangium×A.auriculiformis and Acacia mangium respectively,and the effective data accounted for more than 99%of the original raw reads.Q20 and Q30 are higher than 98%and 95%respectively.After optimized assembly,the total number of transcripts is 133473,the total number of Unigenes is 83117,and the total number of sequence bases is 122735856 bp,of which 63377 are within 1000 bp,accounting for more than 76%of the total.The distribution of Unigenes expression of the three Acacia species is very close,with 20379 Unigenes detected.KEGG function annotation was carried out on these Unigenes,and they were annotated to 129 different pathways,among which the genes related to photosynthesis and plant homone signal transduction pathways were significantly enriched.The expression of Unigenes in map00195(photosynthetic pathway)annotated by three acacia species was analyzed.It was found that the expression of most Unigenes in Acacia mangium×A.auriculiformis was higher than that of their parents.The genes corresponding to these Unigenes are mainly the key genes that affect the photosynthesis process.The expression level of related genes in photosynthetic pathway of Acacia mangium×A.auriculiformis is significantly higher than that of their parents,which is presumed to be the main reason for the growth superiority of Acacia hybrids.This study provides theoretical basis for future of Acacia cross breeding.

关 键 词：相思 杂种优势 转录组测序 基因 光合作用 

分 类 号：S792.99[农业科学—林木遗传育种]