基于16S rDNA基因高通量测序分析农田栽参土壤改良后的细菌群落结构  

Analysis of Bacterial Community Structure of Farmland Planted Ginseng Soil Based on High-throughput Sequencing of 16S rDNA Gene

作　　者：刘晨阳 高成林 赵玥 邹季 许永华[1] Liu Chenyang;Gao Chenglin;Zhao Yue;Zou Ji;Xu Yonghua(College of Traditional Chinese Medicine,Jilin Agricultural University,Changchun,130118;Songyuan Ningjiang Green Food Development Center,Songyuan,138000)

机构地区：[1]吉林农业大学中药材学院,长春130118 [2]松原市宁江区绿色食品开发中心,松原138000

出　　处：《分子植物育种》2021年第5期1731-1740,共10页Molecular Plant Breeding

基　　金：国家重点研发计划课题(2017YFC1702101);吉林省重点科技研发项目(20180201006YY)共同资助。

摘　　要：为揭示农田栽参土壤改良中几种常用肥料施用后对土壤细菌群落的影响。利用Illumina Miseq高通量测序技术,分析测定肥料施用后土壤中细菌群落结构、物种丰度及物种组成。通过相关性分析研究土壤细菌群落多样性与土壤元素及土壤酶活性的关系。结果表明,施用肥料后鞘氨醇单胞菌属(Sphingomonas)、罗思河小杆菌属(Rhodanobacter)、norank_o_Saccharimonadales、节杆菌属(Arthrobacter)、norank_o_Gaiellales等菌属为土壤中优势菌属;F中细菌群落多样性最高,即ace指数与chao1指数最高。处理C细菌群落丰度最高,F处理细菌群落丰度最低;相关性分析发现土壤酶活性、土壤元素与土壤细菌群落结构存在显著相关性,UE、OM、pH、K、Fe、N、Ca等环境因子对土壤细菌结构影响较大。说明除了直接施入菌剂改变土壤细菌群落结构,通过改变土壤性质,也可以间接改变土壤细菌群落结构。研究表明,几种肥料的施用可以改善土壤细菌群落结构,其中,C肥料可以显著提高细菌群落丰度;A处理显著提高有益菌属鞘氨醇单胞菌属(Sphingomonas)的丰度;E处理使拮抗菌属罗思河小杆菌属(Rhodanobacter)丰度增加。本研究为几种常用肥料对土壤改良后土壤细菌种类、群落结构及其功能的影响机制提供实验依据。To reveal the effect of several kinds of fertilizer on soil bacterial community in the field.The bacterial community structure,species richness and species composition in the soil after fertilizer application were analyzed and determined by high-throughput sequencing technology of Illumina Miseq.The relationship between soil bacterial community diversity and soil elements and soil enzyme activity was studied by correlation analysis.The results showed that Sphingomonas,Rhodanobacter,norank_o_Saccharionadales,Arthrobacter and norank_o_Gai-%ellales were the dominant bacteria in the soil,and the diversity of bacterial community in F was the highest,both ACE index and Chao1 index.The results of correlation analysis showed that soil enzyme activity,soil elements and soil bacterial community structure were significantly correlated,and environmental factors such as UE,OM,pH,K,Fe,N,CA had a greater impact on soil bacterial community structure.The results showed that in addition to direct application of fungicides to change the structure of soil bacterial community,through changing soil properties,the structure of soil bacterial community could also be changed indirectly.The results showed that the application of several fertilizers could improve the structure of soil bacterial community,among which C fertilizer could significantly increase the abundance of bacterial community;a treatment could significantly increase the abundance of Sphingomonas;E treatment could increase the abundance of Rhodanobater.This study provides experimental basis for the mechanism of the effects of several common fertilizers on the species,community structure and function of soil bacteria after soil improvement.

关 键 词：农田栽参 土壤改良 高通量测序 土壤细菌群落多样性 

分 类 号：S156[农业科学—土壤学] S154.3[农业科学—农业基础科学]