天麻素对焦虑样胃高敏感性功能性消化不良模型大鼠脑胃调节因子含量的影响  

Effects of gastrodin on brain-stomach modulators in the rat model of functional dyspepsia with anxiety-like gastric hypersensitivity

作　　者：景富春 景璇[3] 张军 王进海[1] 冯诚[1] 杨宝德[4] 胡浩 孙晓明 郑建云[6] 尹晓然[1] JING Fuchun;JING Xuan;ZHANG Jun;WANG Jinhai;FENG Chen;YANG Baode;HU Hao;SUN Xiaoming;ZHENG Jianyun;YIN Xiaoran(Department of Gastroenterology, The Second Affiliated Hospital of Xi’an Jiaotong University, Key Laboratory of Gastrointestinal Motility Disease Research of Shanxi Province, Xi’an 710004, China;Department of Digestive Diseases, Baoji People’s Hospital Affiliated to Yan’an University, Baoji 721000, Shanxi, China;Grade 2018, Class 3, School of Medicine, Shihezi University, Shihezi 832000, Xinjiang, China;Department of Pharmacology, Health Science Center, Xi’an Jiaotong University, Xi’an 710061, China;Basic Medical Experiment Teaching Center, Health Science Center, Xi’an Jiaotong University, Xi’an 710061, China;Department of Pathology, The First Affiliated Hospital of Xi’an Medical University, Xi’an 710004, China)

机构地区：[1]西安交通大学第二附属医院消化内科·陕西省胃肠动力疾病研究重点实验室,陕西西安710004 [2]延安大学附属宝鸡市人民医院消化内科,陕西宝鸡721000 [3]石河子大学医学院,新疆石河子832000 [4]西安交通大学医学部药理学系,陕西西安710068 [5]西安交通大学医学部基础医学实验教学中心,陕西西安710068 [6]西安医学院第一附属医院病理科,陕西西安710077

出　　处：《西部医学》2021年第3期347-351,共5页Medical Journal of West China

基　　金：卫生部部属医院临床学科重点项目(卫规财函[2007]353号)。

摘　　要：目的探讨天麻素对焦虑样胃高敏感性功能性消化不良(FD)模型大鼠脑胃调节因子的影响。方法将40只1日龄SPF级雄性SD仔鼠随机分为对照组、模型组、丁螺环酮组、天麻素(Gas)小剂量组和大剂量组,每组8只。出生第2日起采用序贯应激法,即序贯给予胃刺激和母婴分离,成年后给予束缚应激而制作焦虑样胃高敏感FD大鼠模型。于第8周束缚应激试验结束后开始腹腔注射给予对照组和模型组生理盐水2.0 mL/kg,丁螺环酮组给予丁螺环酮3.125 mg/kg,Gas小、大剂量组分别给予Gas 62.5 mg/kg和125.0 mg/kg,每天1次,连续7天。末次给药后采静脉血,并立即断头处死取脑和胃,用于测定海马、血浆和胃组织中5羟色胺(5-HT)、γ氨基丁酸(GABA)、脑源性神经营养因子(BDNF)和nesfatin-1的含量。结果与对照组相比,模型组大鼠各部位5-HT、BDNF含量和海马内GABA含量均明显减少,而nesfatin-1含量明显增加(P<0.01)。Gas大、小剂量组和丁螺环酮组各部位5-HT、BDNF含量和海马内GABA含量均明显增加,而nesfatin-1含量明显减少,组间比较差异具有统计学意义(P<0.01)。结论Gas能明显增加序贯应激所致焦虑样胃高敏感FD模型大鼠海马、血浆和胃组织中的5-HT、BDNF、GABA的含量,减少nesfatin-1的含量。Objective To investigate the effects of gastrodin on the levels of neurobiochemical modulators shared by brain and stomach in a rat model of FD with anxiety-like gastric hypersensitivity induced by sequential stress.MethodsRats were sequentially subjected to acute gastric irritation(AGI)and maternal separation(MS)early in life and restraint stress(RS)in adulthood.Then they were given intraperitoneal injection of 0.9%saline(2.0 mL/kg,for control and model group),buspirone(3.125 mg/kg,for buspirone group)or Gastrodin(Gas,62.5 or 125.0 mg/kg for Gas low or high dose groups),respectively(one time a day at 8:00 o’clock a.m.for 7 consecutive days).After that blood collection were performed and the rats were finally sacrificed by decapitation.The hippocampus and gastric fundus were promptly isolated and stored at-80℃for ELISA measurements of 5-hydroxytryptamine(5-HT),γ-aminobutyric acid(GABA),brain-derived neurotrophic factor(BDNF)and nesfatin-1.Results Compared with the control group,the hippocampal,plasmic and gastric levels of 5-HT,BDNF and GABA decreased significantly(P<0.01),while the concentrations of nesfatin-1 increased greatly(P<0.01).Gas could significantly attenuate the diminution of the hippocampal,plasmic and gastric levels of 5-HT,BDNF and GABA,but inhibit the increase of nesfatin-1 in the same sites(P<0.01 vs.model rats).Conclusions The results implied that Gas could influence the levels of 5-HT,GABA,BDNF and nesfatin-1 in hippocampus,plasma and gastric tissue of the sequential-stress-treated rats,and could be used in the treatment of FD with anxiety-like gastric hypersensitivity.

关 键 词：天麻素 序贯应激法 胃高敏感性 功能性消化不良 

分 类 号：R285.5[医药卫生—中药学]