亚麻木酚素含量的SSR关联分析  

SSR Correlation Analysis of Linseed Lignan Content

作　　者：伊六喜[1] 萨如拉 王树彦[1] 李志伟[1] 斯钦巴特尔 李强 YI Liuxi;SA Rula;WANG Shuyan;LI Zhiwei;SIQIN Bateer;LI Qiang(Agricultural Collage,Inner Mongolia Agricultural University,Hohhot 010019,China;School of Pharmacy,Baotou Medical College,Inner Mongolia University of Science and Technology,Baotou 014060,China;Inner Mongolia Academy of Agriculture and Husbandry Sciences,Hohhot 010031,China)

机构地区：[1]内蒙古农业大学农学院,内蒙古呼和浩特010019 [2]内蒙古科技大学包头医学院药学院,内蒙古包头014060 [3]内蒙古自治区农牧业科学院,内蒙古呼和浩特010031

出　　处：《华北农学报》2021年第1期63-70,共8页Acta Agriculturae Boreali-Sinica

基　　金：内蒙古自治区高等学校“青年科技英才支持计划”(NJYT-19-B38);内蒙古自然科学基金项目(2020MS03084);内蒙古农业大学高层次人才引进科研启动项目(NDYB2019-8);内蒙古自然科学基金项目(2018LH03030)。

摘　　要：为了进一步揭示亚麻木酚素含量的遗传机制,对220份亚麻核心种质进行2 a 2点重复检测木酚素含量,通过关联分析挖掘木酚素含量相关的SSR标记,为高木酚素含量的亚麻新品种选育提供基础。结果表明,220份亚麻种质木酚素含量变幅为3.24~11.76 mg/g,变异系数为20.34%,广义遗传率61.03%,2 a 2个环境条件下重复检测筛选出7份高木酚素含量亚麻品种。30个SSR引物扩增出203个多态性位点,平均扩增率为56.28%,有效等位基因数平均为1.41,引物多态性含量平均值0.43。群体结构K=4时,220份亚麻种质分为4个群体。203个SSR多态性位点和220份亚麻木酚素含量的关联分析,在GLM模型分析共检测到18个SSR位点,表型解释率为2.07%~13.18%。MLM模型分析共检测到16个位点,表型解释率为3.06%~15.03%,其中LU_83b位点在GLM(13.18%)和MLM(15.03%)下表型解释率均较高,LU_203、LU_661和LU_330位点2个环境条件下均检测到。In order to further reveal the genetic mechanism of the content of flax lignans,220 core collections of flax were repeatedly tested for lignan content for 2 years and 2 points,and SSR markers related to lignan content were mined by association analysis.This study detected SSR loci,and further omics verification can assist genetic improvement of flax lignan content and speed up the breeding process.The results showed that the lignan content of 220 flax germplasms varied from 3.24 to 11.76 mg/g,the coefficient of variation was 20.34%,and the generalized heritability was 61.03%.The 7 flax varieties were selected by repeated testing under 2 environmental conditions for 2 years;30 SSR primers amplified 203 polymorphic sites,with an average amplification rate of 56.28%,and an average number of effective alleles of 1.41.The average value of primer polymorphism content was 0.43.When the group structure K=4,220 flax germplasms were divided into 4 groups.Correlation analysis of 203 polymorphic loci and lignan content,a total of 18 SSR locus were detected by GLM model analysis,and the phenotypic interpretation rate was 2.07%to 13.18%.A total of 16 sites were detected by MLM model analysis,and the phenotypic interpretation rate was 3.06%to 15.03%.Among them,the LU_83b loci had the highest interpretation rate in the GLM(13.18%)and MLM(15.03%)phenotypes,and the LU_203,LU_661,and LU_330 locus were under two environmental conditions.

关 键 词：亚麻 木酚素 SSR标记 关联分析 

分 类 号：S324[农业科学—作物遗传育种]