五步蛇毒对小鼠免疫功能的影响  

Immune Effects of the Deinagkistrodon Acutus Venom in Mice

作　　者：宋天麟 周马林[1] 蒙怡[1] 李亚兰 陶慧娟 廖明[1] 班建东[1] 张学荣[1] SONG Tian-lin;ZHOU Ma-lin;MENG Yi;LI Ya-lan;TAO Hui-juan;LIAO Ming;BAN Jian-dong;ZHANG Xue-rong(Guangxi Medical University,Nanning City,Guangxi Zhuang Autonomous Region,530021,China)

机构地区：[1]广西医科大学,广西南宁530021

出　　处：《蛇志》2021年第1期1-4,共4页Journal of Snake

基　　金：国家自然科学基金资助项目(编号:81360078,81860344)。

摘　　要：目的探索五步蛇毒对正常小鼠免疫功能的影响。方法将KM小鼠随机分为5组,分别为空白组、五步蛇毒安全范围内低剂量组(30 mg/kg)、五步蛇毒安全范围内中剂量组(60 mg/kg)、五步蛇毒安全范围内高剂量组(120 mg/kg)(以下简称五步蛇毒低、中、高剂量组)以及西洋参组(30 mg/kg),每组10只。各组小鼠每日定时灌胃1次,连续14天。观察小鼠的呼吸、精神状态等生命体征。取小鼠脾脏和胸腺组织称重后,计算小鼠脾脏、胸腺指数;制备脾细胞悬液,采用CCK-8法检测小鼠脾淋巴细胞增殖率,碳粒廓清法检测小鼠吞噬系数α,中性红法检测小鼠腹腔巨噬细胞吞噬率;同时采用二硝基氟苯诱导小鼠迟发型变态反应,检测小鼠耳廓肿胀度。结果小鼠的吞噬系数α在五步蛇毒高剂量组最高(5.56±0.46),高于其余四组,差异均有统计学意义(均P<0.05)。小鼠腹腔巨噬细胞吞噬率(%)在五步蛇毒中剂量组和西洋参组中较高,分别为(119.07±19.31)%、(124.79±24.93)%,差异有统计学意义(均P<0.05)。小鼠的B淋巴细胞增殖率(%)在五步蛇毒中剂量组和西洋参组中较高,分别为(105.95±14.59)%、(108.14±10.58)%,差异有统计学意义(均P<0.05)。小鼠的T淋巴细胞增殖率(%)在五步蛇毒中、高剂量组中较高,分别为(119.30±20.07)%、(110.85±23.13)%,差异有统计学意义(均P<0.05)。与空白组比较,五步蛇毒中、高剂量组和西洋参组均能显著提高小鼠的耳廓肿胀度,差异有统计学意义(均P<0.05)。与空白组比较,五步蛇毒各剂量组和西洋参组均能显著提高小鼠脾脏指数(均P<0.05)。与空白组比较,各实验组胸腺指数均无明显差异(均P>0.05)。结论口服低于120 mg/kg的五步蛇毒对正常小鼠的免疫功能具有一定的增强作用。Objective To explore the effects of the Deinagkistrodon acutus venom on the immune function in normal mice.Methods The KM mice were randomly divided into 5 groups:blank group,low-dose group(30 mg/kg)within the safety range of Deinagkistrodon acutus venom,medium-dose group(60 mg/kg)within the safety range of Deinagkistrodon acutus venom,high-dose group(120 mg/kg)within the safety range of Deinagkistrodon acutus venom(hereinafter referred to as low,medium-and high-dose groups of Deinagkistrodon acutus venom)and American ginseng group(30 mg/kg),10 mice in each group.Gave the medicine once a day at regular intervals for 14 days.The vital signs such as respiration and mental state were observed.Took spleen and thymus tissues,calculate spleen and thymus index after weighing;prepared spleen cell suspension,used CCK-8 method to detect lymphocytes proliferation rate;carbon particle clearance method to detect mouse phagocytic coefficientα;the mouse peritoneal macrophage phagocytosis was detected by neutral red assay;dinitrofluorobenzene(DNFB)was used to induce delayed type hypersensitivity in mice and detected the auricle swelling of mice.Results The phagocytic coefficientαof mice in high-dose Agkistrodon acutus venom group was the highest(5.56±0.46),and the differences were statistically significant(all P<0.05).The phagocytosis rate(%)of mice were higher in the medium-dose group and the American ginseng group,which respectively were(119.07±19.31)%and(124.79±24.93)%,the differences were statistically significant(P<0.05).The B lymphocyte proliferation rate(%)of mice were higher in the medium-dose group of and the American ginseng group,which respectively were(105.95±14.59)%and(108.14±10.58)%,the differences were statistically significant(P<0.05).The T lymphocyte proliferation rate(%)of mice were higher in the medium-dose and high-dose groups,which respectively were(119.30±20.07)%and(110.85±23.13)%,the differences were statistically significant(P<0.05).Compared with the blank group,the medium-dose and high-dose grou

关 键 词：五步蛇毒 细胞免疫 体液免疫 非特异性免疫 

分 类 号：R996.3[医药卫生—毒理学]