不同种衣剂对华北大黑鳃金龟幼虫的防治效果及解毒酶活性的影响  

Control efficacies of different kinds of seed-coatings and their effects on the activity of detoxification enzymes in Holotrichia oblita(Coleoptera:Melolonthidae)

作　　者：任美凤 董晋明 李大琪 畅建武[2] 郝晓娟[1] 陆俊姣 REN Meifeng;DONG Jinming;LI Daqi;CHANG Jianwu;HAO Xiaojuan;LU Junjiao(College of Plant Protection,Shanxi Agricultural University,Shanxi Key Laboratory of Integrated Pest Management in Agriculture,Taiyuan 030031,China;Institute of Crop Germplasm Resources,Shanxi Academy of Agricultural Sciences,Taiyuan 030031,China)

机构地区：[1]山西农业大学植物保护学院,农业有害生物综合治理山西省重点实验室,太原030031 [2]山西省农业科学院农作物品种资源研究所,太原030031

出　　处：《植物保护》2021年第2期128-134,共7页Plant Protection

基　　金：国家重点研发计划(2017YFD0201807,2018YFD0200404,2019YFC1710802-5);公益性行业(农业)科研专项(201503124);山西省农业科学院特色农业技术攻关项目(YGG17108);山西省重点研发项目(201803D221012-1)。

摘　　要：为明确不同种衣剂对华北大黑鳃金龟Holotrichia oblita(Coleoptera:Melolonthidae)幼虫的防治效果和对其解毒酶活性的影响,本文通过室内盆栽接虫试验,研究不同种衣剂对红芸豆的安全性和对华北大黑鳃金龟幼虫的防治效果,采用酶动力学法检测不同种衣剂对华北大黑鳃金龟幼虫解毒酶的影响。盆栽试验结果表明:4种种衣剂对红芸豆均安全,显著降低了红芸豆种子被害指数,提高了出苗率和根重;40%溴酰·噻虫嗪悬浮种衣剂(FSC)对华北大黑鳃金龟幼虫防治效果最高,为74.81%,50%氯虫苯甲酰胺FSC次之,为60.65%,二者之间差异不显著。酶动力学结果表明:处理48 h后40%溴酰·噻虫嗪FSC、50%氯虫苯甲酰胺FSC抑制华北大黑鳃金龟幼虫谷胱甘肽S-转移酶活力,72~96 h后与对照相比无显著差异;处理48 h后600 g/L吡虫啉FSC抑制谷胱甘肽S-转移酶活力,96 h后其活性显著高于对照;处理48~72 h后50%吡虫·硫双威FSC对该酶活力的影响与对照相比无显著差异,96 h后其活性显著高于对照。4种种衣剂对羧酸酯酶活力影响均表现为处理48 h后激活,72 h后抑制,96 h后50%吡虫·硫双威FSC和40%溴酰·噻虫嗪FSC对其作用不明显,600 g/L吡虫啉FSC和50%氯虫苯甲酰胺FSC对其有激活作用。To determine the control efficiencies of different seed-coatings on Holotrichia oblita(Coleoptera:Melolonthidae)and their effects on detoxifying enzymes,the pot tests were adopted to determine the safety of these seed-coatings on red kidney beans and their control efficiencies on H.oblita;the enzyme kinetic method was used to detect their effects on the activity of detoxification enzymes in H.oblita.The results of pot tests showed that four kinds of seed-coatings were all safe for the red kidney bean,which significantly increased the emergence rate and root weight and reduced the seed damage index.The control effects of cyantraniliprole·thiamethoxam 40% FSC was most effective(74.81%),followed by chlorantraniliprole 50% FSC(60.65%),without significant difference between the two treatments.The results of enzyme kinetic tests showed that cyantraniliprole·thiamethoxam 40% FSC,chlorantraniliprole 50% FSC inhibited glutathione S-transferase activity 48 h after treatment,but no significant change with control after 72-96 h.Imidacloprid 600 g/L FSC inhibited glutathione S-transferase activity 48 h after treatment but significantly increased glutathione S-transferase activity after 96 h compared with control.Thiodicarb·imidacloprid 50% FSC had no significant difference on glutathione S-transferase activity 48-72 h after treatment compared with control,but significantly increased glutathione S-transferase activity after 96 h compared with control.The activity of carboxyl esterase was induced 48 h after treatment but inhibited 72 h after treated with four kinds of seed-coatings;after 96 h,thiodicarb·imidacloprid 50% FSC and cyantraniliprole·thiamethoxam 40% FSC had no obvious effects,while imidacloprid 600 g/L FSC and chlorantraniliprole 50% FSC had an activation effect.

关 键 词：华北大黑鳃金龟 种衣剂 防治效果 解毒酶 红芸豆 

分 类 号：S435.29[农业科学—农业昆虫与害虫防治]