Effect of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide concentrations on the mechanical and biological characteristics of cross-linked collagen fibres for tendon repair  

作　　者：Zafar Ahmad Jennifer H.Shepherd David V.Shepherd Siddhartha Ghose Simon J.Kew Ruth E.Cameron Serena M.Best Roger A.Brooks John Wardale Neil Rushton 

机构地区：[1]Orthopaedic Research Unit,Department of Surgery University of Cambridge,Addenbrooke’s Hospital,Cambridge,CB20QQ,UK [2]Department of Materials Science and Metallurgy,University of Cambridge,Cambridge,CB30FS,UK [3]Tigenix Ltd,Cambridge,CB40FY,UK

出　　处：《Regenerative Biomaterials》2015年第2期77-85,共9页再生生物材料（英文版）

摘　　要：Reconstituted type I collagen fibres have received considerable interest as tendon implant materials due to their chemical and structural similarity to the native tissue.Fibres produced through a semi-continuous extrusion process were cross-linked with different concentrations of the zerolength cross-linker 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide(EDC)in combination with N-hydroxysuccinimide(NHS).Tensile properties of the fibres were considered,along with imaging of both surface structure and fibrillar alignment.Resistance of the fibres to bacterial collagenase was investigated and fibre sections seeded with human tendon cells for biological characterization,including cell adhesion and proliferation.The work clearly demonstrated that whilst the concentration of EDC and NHS had no significant effect on the mechanics,a higher concentration was associated with higher collagenase resistance,but also provided a less attractive surface for cell adhesion and proliferation.A lower cross-linking concentration offered a more biocompatible material without reduction in mechanics and with a potentially more optimal degradability.

关 键 词：BIOPOLYMER scaffolds soft tissue 

分 类 号：TG1[金属学及工艺—金属学]