miR-146a-5p抑制TRAF6/NF-кB信号通路影响滋养细胞的炎症反应  

miR-146a-5p affects inflammation response of trophoblast by inhibiting TRAF6/NF-κB signaling pathway

作　　者：陈芳荣[1] 吴栋才 陈小菊[1] CHEN Fang-rong;WU Dong-cai;CHEN Xiao-ju(Department of Obstetrics of Hainan General Hospital,Hainan Affifliated Hospital of Hainan Medical University,Haikou 570311,China)

机构地区：[1]海南省人民医院,海南医学院附属海南医院产科,海南海口570311

出　　处：《海南医学院学报》2021年第6期410-415,共6页Journal of Hainan Medical University

基　　金：海南省卫生健康行业科研项目(20A200001);海南省自然科学基金面上项目(817306);海南省高等学校科学研究项目(Hnky2019-40)。

摘　　要：目的:探讨微小核糖核酸(miR)-146a-5p与子痫前期的关系及作用机制。方法:对体外培养绒毛膜癌细胞JEG-3,经脂多糖(LPS)诱导后,与空白对照组相比,检测在LPS诱导的JEG-3细胞中miR-146a-5p和TRAF6的表达。设计分组分为control组、LPS组、miR-146a-5p mimic组、miR-146a-5p inhibitor组。通过QPCR定量检测各组JEG-3细胞中miR-146a-5p以及白细胞介素(IL)-1β、IL-6、IL-8和肿瘤坏死因子-a(TNF-α)的mRNA水平;利用Western Blot检测JEG-3细胞内TRAF6/NF-κB通路相关蛋白的表达。结果:(1)miR-146a-5p mimic组的miR-146a-5p表达明显高于其余3组,差异有统计学意义(P<0.05)。(2)与对照组相比,LPS诱导的JEG-3细胞中miR-146a-5p的表达量增加,TRAF6表达量明显下调(P<0.05)。(3)与对照组比较,加入miR-146a mimic后IL-1β、IL-6、IL-8、TNF-α的mRNA表达水平明显下降(P<0.05),加入miR-146a inhibitor后IL-1β、IL-6、IL-8、TNF-α的mRNA表达水平明显增加(P<0.05),与LPS组相比,差异均无统计学意义(P>0.05)。(4)Western Blot结果显示加入miR-146a mimic后JEG-3细胞内TRAF6、NF-κB蛋白的表达较对照组明显下降;加入miR-146a inhibitor后JEG-3细胞内TRAF6、NF-κB蛋白的表达则明显升高。结论:miR-146-5p可能通过抑制TRAF6/NF-κB信号通路影响子痫前期母胎界面的炎症反应。Objective:To investigate the association of micro-rna(miR)-146a-5p expression with preeclampsia,and further explore the potential mechanism involved.Methods:Compared with the blank control group,the expressions of miR-146a-5p and TRAF6 were detected in lipopolysaccharide(LPS)-induced JEG-3 cells.Chorionic carcinoma cell JEG-3 in vitro culture are divided into control,miR-146a-5p mimic+lipopolysaccharide(LPS),miR-146a-5p mimic and miR-146a-5p inhibitor groups.qRT-PCR analysis were used to detect the mRNA of miR-146a-5p,IL-1β,IL-6,IL-8 and TNF-α.Western blot assays were carried out to determine the protein expression of TRAF6/NF-κB pathway related proteins.Results:(1)miR-146a-5p expression in miR-146a mimic-5p group were significantly higher than the other three groups(P<0.05).(2)Compared with the control group,the expression level of miR-146a-5p in JEG-3 cells induced by LPS was significantly increased,and the expression level of TRAF6 was significantly reduced(P<0.05).(3)Compared with the control group,the mRNA expression levels of IL-1β,IL-6,IL-8,and TNF-αdecreased significantly after using miR-146a mimic(P<0.05).After adding miR-146a inhibitor,the mRNA expression levels of IL-1β,IL-6,IL-8,and TNF-αwere significantly increased(P<0.05).However,compared with the mimic+LPS group,the difference was not statistically significant(all P>0.05).(4)The results of Western blot showed that the expression of TRAF6 and NF-κB protein in JEG-3 cells decreased significantly after adding miR-146a mimic and increased after adding miR-146a inhibitor.Conclusion:MiR-146-5p can affect the inflammation response of maternal-fetal interface by inhibiting TRAF6/NF-κB signaling pathway in patients with preeclampsia.

关 键 词：miR-146-5p TRAF6/NF-κB信号通路 滋养细胞 炎症反应 

分 类 号：R714.244[医药卫生—妇产科学]