Asymmetric bioreduction of γ- and δ-keto acids by native carbonyl reductases from Saccharomyces cerevisiae  

作　　者：Chunlei Ren Tao Wang Xiaoyan Zhang Jiang Pan Jianhe Xu Yunpeng Bai 

机构地区：[1]State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shanghai 200237,China [2]Shanghai Collaborative Innovation Centre for Biomanufacturing,East China University of Science and Technology,Shanghai 200237,China

出　　处：《Chinese Journal of Chemical Engineering》2021年第1期305-310,共6页中国化学工程学报（英文版）

基　　金：financially sponsored by the National Key Research and Development Program of China (2016YFA0204300, 2019YFA09005000);the National Natural Science Foundation of China (21536004, 21776085, 21871085);the Natural Science Foundation of Shanghai (18ZR1409900);Key Project of the Shanghai Science and Technology Committee (18DZ1112703);the Fundamental Research Funds for the Central Universities (WF1714026)。

摘　　要：Optically pure(R)-γ-and(R)-δ-lactones can be prepared by intramolecular cyclization of chiral hydroxy acids/esters reduced asymmetrically from γ-and δ-keto acids/esters using Saccharomyces cerevisiae(S.cerevisiae) as a whole-cell biocatalyst.However,some of the enzymes catalyzing these reactions in S.cerevisiae are still unknown up to date.In this report,two carbonyl reductases,OdCRl and OdCR2,were successfully discovered,and cloned from S.cerevisiae using a genome-mining approach,and overexpressed in Escherichia coli(E.coli).Compared with OdCR1,OdCR2 can reduce 4-oxodecanoic acid and 5-oxodecanoic acid asymmetrically with higher stereoselectivity,generating(R)-γ-decalactone(99% ee) and(R)-δ-decalactone(98% ee) in 85% and 92%yields,respectively.This is the first report of native enzymes from S.cerevisiae for the enzymatic synthesis of chiral γ-and δ-lactones which is of wide uses in food and cosmetic industries.

关 键 词：Keto acids/esters (R)-γ-/δ-Decalactones Carbonyl reductase Asymmetric reduction Saccharomyces cerevisiae 

分 类 号：TQ235[化学工程—有机化工]