四妙丸、六君子丸干预高尿酸血症合并胰岛素抵抗大鼠模型的实验研究  

Effect of Simiao Pill and Liujunzi Pill on rat model of hyperuricemia complicated with insulin resistance

作　　者：杨淑芬 徐隽斐[2] 陈文浩 胥晓芳[1] 姚静慧 沈佳莹 杨光辉[1] 潘新[1] YANG Shufen;XU Junfei;CHEN Wenhao;XU Xiaofang;YAO Jinghui;SHEN Jiaying;YANG Guanghui;PAN Xin(Department of Rheumatology,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200021,China;Department of Endocrinology,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200021,China;Department of Nephrology,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200021,China)

机构地区：[1]上海中医药大学附属曙光医院风湿免疫科,上海200021 [2]上海中医药大学附属曙光医院内分泌科,上海200021 [3]上海中医药大学附属曙光医院肾病科,上海200021

出　　处：《上海中医药杂志》2021年第3期81-86,共6页Shanghai Journal of Traditional Chinese Medicine

基　　金：上海中医药大学高峰高原学科基金(临床人才专项)。

摘　　要：目的研究四妙丸、六君子丸对高脂饲料联合果糖水喂养、氧嗪酸钾灌胃所致的高尿酸血症(HUA)合并胰岛素抵抗(IR)大鼠的影响。方法Wistar雄性大鼠36只,空白组6只(不予干预),其余大鼠建立HUA合并IR模型,造模成功后随机分为模型组、苯溴马隆组(苯溴马隆0.83 g/kg)、非布司他组(非布司他1.24 g/kg)、四妙丸组(四妙丸1.38 g/kg)、六君子丸组(六君子丸1.73 g/kg),每组6只。给药4周后称量各组大鼠体质量及腹腔脂肪的质量,并检测各组血尿酸(UA)、胰岛素(INS)、IR指数(HOMA-IR)、血糖(FPG)、血脂四项[总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白(LDL-c)、高密度脂蛋白(HDL-c)]、尿素氮(BUN)、肌酐(SCr)、肝脏黄嘌呤氧化酶(XOD)活性、肾脏尿酸盐重吸收转运子(URAT1)表达。结果(1)与空白组比较,模型组和各给药组的体质量、腹腔脂肪质量均明显增加(P<0.01),四妙丸组、六君子丸组大鼠腹腔脂肪质量较模型组、苯溴马隆组、非布司他组明显减少(P<0.01)。(2)与空白组比较,模型组UA、INS、FPG、HOMA-IR水平均显著升高(P<0.05,P<0.01);与模型组比较,各给药组UA、INS、HOMA-IR水平均降低(P<0.05,P<0.01),其中非布司他组UA降低最为显著,四妙丸组与苯溴马隆组HOMA-IR减少最明显(P<0.01)。(3)各组大鼠血脂、BUN、SCr水平差异均无统计学意义(P>0.05)。(4)与模型组比较,四妙丸组、非布司他组抑制XOD活性(P<0.05)。(5)与模型组比较,四妙丸、六君子丸、苯溴马隆能下调URAT1的表达(P<0.01)。结论四妙丸、六君子丸具有降尿酸、改善IR的作用,为健脾清热利湿法治疗HUA合并IR提供思路。Objective To investigate the therapeutic effects of Simiao Pill and Liujunzi Pill on hyperuricemic(HUA)rats with insulin resistance(IR)induced by high fat diet combined with fructose water feeding and potassium oxazinate intragastric administration.Methods Of the 36 male Wistar rats,6 were in the blank group(without intervention)and the others were established the model of HUA combined with IR.After successful modeling,they were randomly divided into 5 groups:model group,benzbromarone group(benzbromarone 0.83 g/kg),febutastat group(febutastat 1.24 g/kg),Simiao Pill group(Simiao Pill 1.38 g/kg),Liujunzi Pill group(Liujunzi Pill 1.73 g/kg),with 6 rats in each group.After 4 weeks of drug administration,the body weight and the abdomin-fat weight of the rats were recorded,and indexes including serum uric acid(UA),insulin(INS),insulin resistance index(HOMA-IR),fasting serum glucose(FPG)fasting serum lipid[total cholesterol(TC),triglyceride(TG),low density lipoprotein(LDL-c),high density lipoprotein(HDL-c)],urea nitrogen(BUN),creatinine(SCr),liver xanthine oxidase(XOD)activity and renal uric acid transporter URAT1 expression were detected.Results(1)The body weight and abdomin-fat weight of the model group and the administration groups were significantly higher than those of the blank group(P<0.01),while the abdominal-fat of Simiao Pill group and Liujunzi Pill group was significantly lower than that of the model group,benzbromarone group and febutastat group.(2)UA,INS,FPG and HOMA-IR in the model group were significantly higher than those in the blank group(P<0.05,P<0.01).The levels of UA,INS and HOMA-IR in the administration groups were lower than those in the model group(P<0.05,P<0.01).Among them,the decrease of UA was most significant in the febuxostat group(P<0.01)and the reduction of HOMA-IR was most significant in the Simiao Pill and the benzbromarone group(P<0.01).(3)There were no significant difference in serum lipids,BUN,and SCr among all groups(P>0.05).(4)Compared with the model group,Simiao Pill and febuxostat

关 键 词：高尿酸血症 胰岛素抵抗 大鼠 四妙丸 六君子丸 

分 类 号：R285.5[医药卫生—中药学]