Biomolecular functionalization for enhanced cell–material interactions of poly(methyl methacrylate)surfaces 5th China-Europe Symposium on Biomaterials in Regenerative Medicine(CESB 2015)Hangzhou,China April 7–10,2015  

作　　者：Xavier Punet Rodolphe Mauchauffe´ Jose´C.Rodrı´guez-Cabello Matilde Alonso Elisabeth Engel Miguel A.Mateos-Timoneda 

机构地区：[1]Biomaterials for Regenerative Therapies Group,Institute for Bioengineering of Catalonia(IBEC),Barcelona 08028,Spain [2]CIBER en Bioingenerı´a,Biomateriales y Nanomedicina(CIBER-BBN),Spain [3]G.I.R.Bioforge,Universidad Valladolid(UVA),Valladolid 47011,Spain [4]Department of Material Science and Metallurgical Engineering,Technical University of Catalonia(UPC),Barcelona 08028,Spain

出　　处：《Regenerative Biomaterials》2015年第3期167-175,共9页再生生物材料（英文版）

基　　金：This work was supported by the Spain’s Ministerio de Economı´a y Competitividad[projects MAT2008-06887-C03-01,MAT2010-15310,MAT2013-41723-R,MAT2013-42473-R,VA313U14 and VA244U13].X.P.acknowledges grant BES-2009-027524 from the Spain’s Ministerio de Economı´a y Competitividad.

摘　　要：The integration of implants or medical devices into the body tissues requires of good cell–material interactions.However,most polymeric materials used for these applications lack on biological cues,which enhanced mid-and long-term implant failure due to weak integration with the surrounding tissue.Commonly used strategies for tissue–material integration focus on functionalization of the material surface by means of natural proteins or short peptides.However,the use of these biomolecules involves major drawbacks such as immunogenic problems and oversimplification of the constructs.Here,designed elastin-like recombinamers(ELRs)are used to enhance poly(methyl methacrylate)surface properties and compared against the use of short peptides.In this study,cell response has been analysed for different functionalization conditions in the presence and absence of a competing protein,which interferes on surface–cell interaction by unspecific adsorption on the interface.The study has shown that ELRs can induce higher rates of cell attachment and stronger cell anchorages than short peptides,being a better choice for surface functionalization.

关 键 词：cell adhesion elastin-like recombinamer poly(methyl methacrylate) PMMA RGD peptide surface modification 

分 类 号：O63[理学—高分子化学]