嘉氏羊蹄甲组织培养体系的建立  

Establishment of Tissue Culture System of Bauhinia galpinii

作　　者：何超银 魏丹[1] 唐洪辉[1] 许冲勇[2] 蓝燕群[1] 冯志坚[2] 陈友光 张耕 HE Chaoyin;WEI Dan;TANG Honghui;XU Chongyong;LAN Yanqun;FENG Zhijian;CHEN Youguang;ZHANG Geng(Guangdong Provincial Key Laboratory of Silviculture,Protection and Utilization/Guangdong Academy of Forestry,Guangzhou,Guangdong 510520,China;College of Forestry and Landscape Architecture,South,China Agricultural University,Guangzhou,Guangdong 510642,China;Guangdong Ruchun Ecological Group Co,Ltd,Guangzhou,Guangdong 510530,China)

机构地区：[1]广东省森林培育与保护利用重点实验室/广东省林业科学研究院,广东广州510520 [2]华南农业大学,广东广州510642 [3]广东如春生态集团有限公司,广东广州510530

出　　处：《林业与环境科学》2021年第1期110-115,共6页Forestry and Environmental Science

基　　金：广州市科技计划项目(201904010397)。

摘　　要：以嘉氏羊蹄甲Bauhinia galpinii带腋芽茎段为外植体,建立组织培养快繁体系。结果表明:最适合的嘉氏羊蹄甲带腋芽茎段诱导的培养基为:MS+0.5 mg·L^(-1) 6-BA+0.05 mg·L^(-1) NAA,诱导率为80.00%;最佳增殖培养基为:WPM+0.50 mg·L^(-1) 6BA+0.40 mg·L^(-1)IAA,增殖系数达4.62;最佳生根培养基为:1/2MS+1.00 mg·L^(-1) IBA +0.50 mg·L^(-1)NAA+0.10 mg·L^(-1) IAA +15 g·L^(-1) 蔗糖+7 g·L^(-1)卡拉胶,生根率达98.00%;最佳移栽基质为:V(黄心土):V(泥炭):V(珍珠岩)=1:3:1,试管苗移栽成活率达86.7%。试验建立了嘉氏羊蹄甲组织培养体系,提高了嘉氏羊蹄甲增殖率、生根率和移栽成活率。The tissue culture system of Bauhinia galpinii with axillary buds was established.The results showed that the impact of.the most suitable medium for the induction of stem segment with axillary bud was MS+0.5 mg·L^(-1)6-BA+0.05 mg·L^(-1)NAA,the induction rate was 80.00%;the best proliferation medium was WPM+0.50 mg·L^(-1)6BA+0.40 mg·L^(-1) IAA,the proliferation coefficient was 4.62;the best rooting medium was 1/2 ms+1.00 mg·L^(-1) IBA+0.50 mg·L^(-1) NAA+0.10 mg·L^(-1) IAA+15 g·L^(-1) sucrose+7 g·L^(-1) carrageenan,the rooting rate was 98.00%;the best transplanting medium was V(yellow soil):V(peat):V(perlite)=1:3:1,the survival rate of plantlets transplanting was 86.7%.The tissue culture system was established,which could improve the proliferation rate,rooting rate and transplanting survival rate.

关 键 词：嘉氏羊蹄甲 组织培养 正交试验 增殖培养 

分 类 号：Q944.6[生物学—植物学]