Purification,structural elucidation,and in vitro antitumor effects of novel polysaccharides from Bangia fuscopurpurea  

作　　者：Jingna Wu Xiaoting Chen Kun Qiao Yongchang Su Zhiyu Liu 

机构地区：[1]Xiamen Key Laboratory of Marine Medicinal Natural Products Resources,Xiamen Medical College,361023,Xiamen,China [2]Fujian Universities and Colleges Engineering Research Center of Marine Biopharmaceutical Resources,Xiamen Medical College,361023,Xiamen,China [3]Fisheries Research Institute of Fujian,361013,Xiamen,China

出　　处：《Food Science and Human Wellness》2021年第1期63-71,共9页食品科学与人类健康（英文）

基　　金：the Science and Technology Project of Xiamen Medical College(K2016-36).

摘　　要：A water-soluble polysaccharide,designated BFP-3,was isolated from Bangia fuscopurpurea by hot water extraction,anion-exchange,and size-exclusion chromatography and tested to determine its antitumor activity.The structural characteristics of BFP-3 were investigated by chemical and spectroscopic methods,including partial acid hydrolysis,methylation analysis,one-and two-dimensional nuclear magnetic resonance,and gas chromatography-mass spectrometry.The results showed that BFP-3 was mainly comprised of rhamnose,arabinose,mannose,glucose,and galactose.Moreover,the weight-average molecular weight of BFP-3 was estimated to be approximately 333 kDa.The backbone of BFP-3 was primarily composed of repeating 5-α-l-Araf-1→(4-α-d-Glcp-1)_(4)→4,6-β-d-Manp-1 units,and the side chains consisted of repeatingβ-d-Galp-1→(4-β-d-Galp-1)_(4)→4,6-β-d-Galp-1→3,4-α-l-Rhap,β-l-Arap-1→(3-β-d-Galp-1)_(3),andβ-l-Arap-1 units.Counting Kit-8 assays revealed that BFP-3 significantly inhibited the proliferation of A2780,COC1,SKOV3,HO-8910,and OVCAR3 ovarian cancer cells in vitro,indicating that BFP-3 could have potential applications in the treatment of ovarian cancer.

关 键 词：Bangia fuscopurpurea POLYSACCHARIDE Structure ovarian cancer Antitumor effect 

分 类 号：TQ464.1[化学工程—制药化工]