益母草碱抑制NLRP3炎症小体过度激活调控巨噬细胞M1/M2表型分化  

Effect of leonurine on peritoneal macrophages M1/M2 phenotypic differentiation via inhibiting overactivation of NLRP3 inflammasome

作　　者：华荣 陈瑶[1] HUA Rong;CHEN Yao(Department of Pharmacy,Taizhou People’s Hospital,Taizhou 225300,China)

机构地区：[1]泰州市人民医院药学部临床药学科,江苏泰州225300

出　　处：《药学实践杂志》2021年第2期143-147,共5页Journal of Pharmaceutical Practice

摘　　要：目的研究益母草碱对脂多糖(LPS)诱导小鼠腹腔巨噬细胞免疫应答影响及相关机制。方法分离小鼠腹腔巨噬细胞,用脂多糖和益母草碱预处理24 h,MMT法检测巨噬细胞活性;Griess法检测NO释放量;ELISA法检测IL-1β、IL-18、IL-6、TNF-α的释放量;RT-PCR法检测NLRP3、ASC、caspase-1、TNF-α、iNOS、Arg-1和CD206的mRNA表达量;Western blot检测NLRP3、ASC、caspase-1蛋白表达量。结果益母草碱能显著抑制脂多糖引起的巨噬细胞上清液中NO、IL-1β、IL-18、IL-6、TNF-α的释放。RT-PCR及Western blot实验结果显示,益母草碱可以抑制脂多糖引起的巨噬细胞中NLRP3、ASC、caspase-1的mRNA及蛋白表达;益母草碱还能明显抑制脂多糖所诱导的巨噬细胞向M1型分化,并促进巨噬细胞向M2型分化。结论益母草碱能通过抑制NLRP3炎症小体,促进脂多糖诱导的巨噬细胞由M1表型向M2表型分化。Objective To find the effect of leonurine on LPS-induced macrophages activation and its potential mechanism.Methods Mouse primary peritoneal macrophages were isolated and pretreated for 24 h with LPS and leonurine.MTT assay was used to detect the cell viability of macrophages.The production of IL-1β,IL-6,TNF-αand IL-18 in culture medium were tested by ELISA,and the production of NO was detected by Griess reagent.The mRNA expression of NLRP3,ASC,caspase-1,TNF-α,iNOS,Arg-1 and CD206 were detected by RT-PCR,and the protein expression of NLRP3,ASC and caspase-1 were detected by Western blotting.Results LPS can significantly increase the releases of NO、IL-1β、IL-6、TNF-αand IL-18 from macrophages.Leonurine can suppress the expression of pro-inflammatory factor levels,such as IL-1β(P<0.05),IL-18(P<0.05),NO(P<0.05),IL-6(P<0.05)and TNF-α(P<0.05).Leonurine can decrease the activation of macrophage as well as the expression of NLRP3 Inflammasome.Protein expressions of NLRP3、ASC、caspase-1 were mitigated.Conclution Leonurine exerts beneficial effects through M1/M2 phenotypic differentiation of peritoneal macrophage via inhibiting overactivation of NLRP3 inflammasome.These findings suggest that leonurine might have a therapeutic potential for pelvic inflammatory disease.

关 键 词：益母草碱 脂多糖 巨噬细胞 NLRP3炎症小体 M1/M2表型 

分 类 号：R965[医药卫生—药理学]