DNA条形码技术结合形态学特征鉴定中国一新纪录种-艾弗斯曼多眼蝶  

DNA Bar-Coding Combined with Morphological Characteristics to Identify a New Record Species:Kirinia eversmanni from China

作　　者：郭文韬 努日耶·木合太尔 罗顺刚 钟问 胡红英 GUO Wentao;Nuriye Muhetaier;LUO Shungang;ZHONG Wen;HU Hongying(College of Life Science and Technology,Xinjiang University/Xinjiang Key Laboratory of Biological Resources and Genetic Engineering,Urumqi 830046,China)

机构地区：[1]新疆大学生命科学与技术学院/新疆生物资源基因工程重点实验室,乌鲁木齐830046

出　　处：《新疆农业科学》2021年第3期547-555,共9页Xinjiang Agricultural Sciences

基　　金：国家重点研发计划“天山野果林关键生物类群生存现状与有害生物谱系特征”(2016YFC0501501);环境保护部生物多样性保护专项“全国蝴蝶多样性观测(新疆阜康市、吉木萨尔县和霍城县观测样区)”(SDZXWJZ01012)。

摘　　要：【目的】利用DNA条形码技术与形态学相结合鉴定中国新纪录种艾弗斯曼多眼蝶Kirinia eversmanni,为其准确鉴定,以及开展该蝴蝶生物学、生态学研究提供参考。【方法】以艾弗斯曼多眼蝶成虫腹部为材料提取基因组DNA,线粒体细胞色素氧化酶I(Cytochrome Oxidase I,COI)基因作为通用引物进行PCR扩增,将其序列作为艾弗斯曼多眼蝶的DNA条形码,并解剖艾弗斯曼多眼蝶的雄性外生殖器,描述其雄性外生殖器的形态结构结合其翅面特征。【结果】艾弗斯曼多眼蝶的COI基因序列长度为696 bp,与NCBI中已知序列相似性最高可达99.70%,研究物种与NCBI中已有纪录种Kiriniaeversmanni为同一物种。对艾弗斯曼多眼蝶翅面特征以及雄性外生殖器的阳茎、背兜及抱器瓣等结构进行描述形态学特征与获得的序列相结合可作为艾弗斯曼多眼蝶的多个鉴别特征。【结论】利用DNA条形码序列结合形态学描述使得对艾弗斯曼多眼蝶的鉴定结果更加全面准确。【Objective】Kirinia eversmanni,a newly recorded species in China,is identified by DNA bar-coding combined with morphology,which is likely to provide a research basis for its accurate identification and a reference for the study of butterfly’s biology and ecology.【Methods】The Kirinia eversmanni adult abdomen was used to extract genomic DNA,mitochondrial Cytochrome Oxidase I(COI)gene was applied as a general purpose primer for PCR amplification,the sequence as its DNA barcode to anatomize male genitalia and describe the characteristics of male genitalia morphological structure and the surface of the wing.【Result】The COI gene sequence length of Kirinia eversmanni was 696 bp,and the similarity of sequence between Kirinia eversmanni and the known sequences in NCBI was up to 99.70%,which could prove that the species studied was the same one as the species recorded in NCBI,Kirinia eversmanni,and also reflected the authenticity and reliability of the experimental sequence.At the same time,the morphological characteristics of aedeagus,tegumen and valvae structures of male genitalia were described and combined with the obtained sequences,which could be used for accurate identification evidence of Kirinia eversmanni.【Conclusion】DNA bar-coding sequence combined with morphological description makes the identification result of Kirinia eversmanni more comprehensive and accurate.

关 键 词：COI基因序列 雄性外生殖器 物种鉴定 整合分类学 

分 类 号：S188[农业科学—农业基础科学]