绵羊Myostatin前肽对成肌细胞的分化作用  

Effects of Sheep Myostatin Propeptide on Myoblast Differentiation

作　　者：杜玮[1] 夏俊[1] 刘明军[1] 张杨[1] DU Wei;XIA Jun;LIU Mingjun;ZHANG Yang(Research Insititute of Animal Sciences,Xinjiang Academy of Animal Sciences,Urumqi 830000,China)

机构地区：[1]新疆畜牧科学院畜牧研究所,乌鲁木齐830000

出　　处：《新疆农业科学》2021年第3期581-588,共8页Xinjiang Agricultural Sciences

基　　金：杰出青年科技人才培养项目“Myostatin成熟肽核酸疫苗的构建及对骨骼肌生长的影响”;自治区天山英才工程;自治区公益性科研院所基本科研业务经费“新疆褐牛健康养殖技术集成研究”(XY2019115)。

摘　　要：【目的】研究绵羊Myostatin前肽对C2C12细胞分化的影响。【方法】构建并包装Myostatin前肽基因过表达慢病毒质粒,包装慢病毒,分别用LV-CMV-MSTN-flag-GFP和LV-CMV-GFP慢病毒感染C2C12细胞,检测重组慢病毒对C2C12细胞的感染能力。【结果】LV-CMV-GFP感染组可见明显的长条状肌管,LV-CMV-MSTN-flag-GFP组只见少量肌管的形成,肌管融合率的测定。未处理组,对照组,试验组肌管融合率分别为5.53%、6.62%、9.38%,试验组即转染Myostatin前肽的肌管融合率显著高于对照组和未处理组(P<0.05)。【结论】Myostatin前肽基因的过表达抑制了Myostatin基因的表达,影响肌管的形成,抑制了分化的进程。【Objective】To study the effect of sheep myostatin propeptide on myoblast differentiation.【Method】Myostatin cDNA Lentivirus vector plasmid was constructed and expressed in C2C12 cell and the ability of myostatin prepeptide to infect C2C12 cells was studied.【Result】The LV-CMV-GFP infection group showed obvious long strips of myotubes,while LV-CMV-MSTN-flag-GFP group saw only a small amount of myotubes formed.To further determine the differentiation of myoblasts,myotube fusion rate was determined.The myotube fusion rates of were 5.53%,6.62%and 9.38%respectively in the untreated group,the control group and the test group.The fusion rate of myotubes in the test group was significantly higher than that in the control group and the untreated group(P<0.05).【Conclusion】The overexpression of Myostatin pro-peptide gene inhibited the expression of Myostatin gene,thus affecting the formation of myotubes and inhibiting the process of differentiation.

关 键 词：Myostatin前肽 C2C12细胞 肌管融合率 

分 类 号：S826[农业科学—畜牧学] S188[农业科学—畜牧兽医]