HPLC法测定磷酸替米考星可溶性粉中替米考星含量的研究  

HPLC Method for Determining Content of Tilmicosin inTilmicosin Phosphate Soluble Powder

作　　者：马春芳 李敏[1] 张雯 吴春燕[1] 陈建蓉[1] 邓亚婷 王旗 余永涛[2] 杨奇 MA Chun-fang;LI Min;ZHANG Wen;WU Chun-yan;CHEN Jian-rong;DENG Ya-ting;WANG Qi;YU Yong-tao;YANG Qi(Ningxia Supervision Institue for Veterinary Drugs and Administration,Yinchuan 750011,China;College of Agriculture,Ningxia University,Yinchuan 750021,China)

机构地区：[1]宁夏回族自治区兽药饲料监察所,银川750011 [2]宁夏大学农学院,银川750021

出　　处：《中国兽药杂志》2021年第3期17-24,共8页Chinese Journal of Veterinary Drug

基　　金：宁夏回族自治区重点研发计划项目“畜禽产品质量安全评价与监控技术应用研究”(2018BBF02002)。

摘　　要：建立了磷酸替米考星可溶性粉中替米考星含量的HPLC测定方法。采用高效液相色谱(HPLC)法,使用十八烷基硅烷键合硅胶为填充剂的色谱柱,以水∶乙腈∶磷酸二丁胺缓冲液∶四氢呋喃(805∶115∶25∶55)为流动相,流速1.0 mL/min,检测波长280 nm,柱温25℃。按外标法以峰面积计算含量。在此色谱条件下,替米考星顺式、反式异构体峰分离度良好,磷酸替米考星可溶性粉的线性范围为0.2~0.8 mg/mL(R 2=0.9998),平均回收率为97.96%,RSD<1.0%。本研究建立的方法分离效果好、精密度高、稳定性好,可作为磷酸替米考星可溶性粉的质量关键控制指标。To establish a HPLC method for the determination in tilmicosin phosphate soluble powder,HPLC was used for the column with octadecyl silane bonded silica gel as the filler,and the mobile phase was determined with water∶acetonitrile∶dibutylamine phosphate solution∶tetrahydrofuran(805∶115∶25∶55);The flow rate was 1.0 mL/min,the detection wavelength was 280 nm,and the column temperature was 25℃calculating content by peak area according to external standard method.Results showed that under these chromatographic conditions,the separation of cis-and trans-isomers was satisfactory.The linear range of tilmicosin phosphate soluble powder was 0.2~0.8 mg/mL,R 2=0.9998.The average recovery was 97.96%,RSD<1.0%.The method established in this study has good separation effect,high precision and good stability,and can be used as a key quality control index for the tilmicosin phosphate soluble powder.

关 键 词：磷酸替米考星可溶性粉 高效液相色谱法 含量测定 

分 类 号：S859.83[农业科学—临床兽医学]