醉马草中黄酮类化学成分研究  

Flavonoids chemical constituents from Achnatherum inebrians

作　　者：苑祥 李震源[1] 梅丽娟 张培成[1] YUAN Xiang;LI Zhen-yuan;MEI Li-juan;ZHANG Pei-cheng(State Key Laboratory of Bioactive Substance and Function of Natural Medicines,Institute of Materia Medica,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100050,China;Qinghai Provincial Key Laboratory of Tibetan Medicine Research,Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining 810008,China)

机构地区：[1]中国医学科学院北京协和医学院药物研究所,天然药物活性物质与功能国家重点实验室,北京100050 [2]中国科学院西北高原生物研究所,青海省藏药研究重点实验室,青海西宁810008

出　　处：《中草药》2021年第4期937-942,共6页Chinese Traditional and Herbal Drugs

基　　金：“重大新药创制”科技重大专项(2018ZX09711001-001);国家自然科学基金项目(81773588);博士后科学基金项目(2019M650579);中国医学科学院医学与健康科技创新工程(2016-I2M-1-010)。

摘　　要：目的研究禾本科芨芨草属植物醉马草Achnatheruminebrians中的化学成分。方法综合运用HP-20大孔树脂、ODS中压色谱、SephadexLH-20凝胶柱色谱以及制备型高效液相色谱等方法进行系统的分离纯化,根据化合物的理化性质及其波谱数据,并通过对比文献报道的波谱数据,鉴定化合物的化学结构。结果从醉马草乙醇提取物中分离得到15黄酮类化合物,分别鉴定为异红草素(1)、金丝桃苷(2)、槲皮素-3-O-β-D-吡喃葡萄糖基-(3′→O-3′′′)-槲皮素-3?-O-β-D-吡喃半乳糖苷(3)、3?-甲氧基槲皮素-3-O-α-L-吡喃鼠李糖基-(1→6)-β-D-吡喃葡萄糖苷(4)、异鼠李素-3-O-α-L-吡喃鼠李糖基-(1→2)-β-D-吡喃葡萄糖苷(5)、槲皮素-3-O-α-L-吡喃鼠李糖基-(1→2)-β-D-吡喃葡萄糖苷(6)、槲皮素-3-O-β-D-吡喃葡萄糖基-7-O-α-L-吡喃鼠李糖苷(7)、山柰酚-3-O-α-L-吡喃鼠李糖基-(1→2)-β-D-吡喃葡萄糖苷(8)、山柰酚-3-O-α-L-吡喃鼠李糖基-(1→4)-β-D-吡喃葡萄糖苷(9)、8-甲氧基槲皮素-3-O-β-吡喃葡萄糖苷(10)、槲皮素-3-O-α-L-吡喃鼠李糖基-(1→2)-β-D-吡喃半乳糖基-7-O-β-D-吡喃葡萄糖苷(11)、5,7,3?-三羟基-8,4?,5?-三甲氧基黄酮(12)、木犀草素-7-O-葡萄糖醛酸苷-6??-甲酯(13)、木犀草素-7-O-葡萄糖醛酸苷(14)和金圣草黄素(15)。结论所有化合物均为首次从醉马草中分离得到。Objective To investigate the chemical constitutes from Achnatherum inebrians. Methods The compounds were isolated and purified by a combination of various chromatographic techniques including HP-20 macroporous resin, ODS MPLC, Sephadex LH-20, and semi-preparative HPLC. Their structures were identified by spectroscopic data and comparing the spectral data reported in literature. Results Fifteen compounds were isolated and identified from the ethanol extract of A. inebrians and their structures were elucidated as isoorientin(1), hyperoside(2), quercetin-3-O-β-D-glucopyranoside(3′→O-3′′′)quercetin-3-O-β-Dgalactopyranoside(3), 3’-O-methylquercetin-3-O-α-L-rhamnopyranosyl(1→6)-β-D-glucopyranoside(4)、isorhamnetin-3-O-α-Lrhamnopyranosyl(1→2)-β-D-glycopyranoside(5), quercetin-3-O-α-L-rhamnopyranosyl(1→2)-β-D-glucopyranoside(6), quercetin-3-O-β-D-glucopyranoside-7-O-α-L-rhamnopyranoside(7), kaempferol 3-O-α-L-rhamnopyranosyl(1→2)-β-D-glucopyranoside(8), kaempferol 3-O-α-L-rhamnopyranosyl(1→4)-β-D-glucopyranoside(9), 8-methoxyquercetin 3-O-β-glucoside(10), quercetin-3-O-α-L-rhamnopyranosyl(1→2)-β-D-galactopyranoside-7-O-β-D-glucopyranoside(11), 5,7,3-trihydroxy-8,4,5-trimethoxyflavone(12), luteolin-7-O-glucuronide-6’’-methyl ester(13), luteolin-7-O-glucuronide(14) and chrysoeriol(15). Conclusion All compounds are isolated from this plant for the first time.

关 键 词：醉马草 黄酮 异红草素 金丝桃苷 木犀草素-7-O-葡萄糖醛酸苷 金圣草黄素 

分 类 号：R284.1[医药卫生—中药学]