基于传统煎药工艺的黄芪药材HPLC指纹图谱及化学计量学质量评价研究  

Fingerprints and quality evaluation of Astragali Radix by HPLC coupled with chemometrics based on traditional decoction process

作　　者：张倩[1] 杨怀瑾 周悦[1] 余亦婷 庄欣雅 毛春芹[1] 谢辉[1] 李林[1] 陆兔林[1] 严国俊[1] ZHANG Qian;YANG Huai-jin;ZHOU Yue;YU Yi-ting;ZHUANG Xin-ya;Mao Chun-qin;XIE Hui;LI Lin;LU Tu-ling;YAN Guo-jun(School of Pharmacy,Nanjing University of Chinese Medicine,Nanjing 210023,China;Nan jing MoChou Vocational School,Nanjing 210017,China)

机构地区：[1]南京中医药大学药学院,江苏南京210023 [2]南京市莫愁中等专业学校,江苏南京210017

出　　处：《中草药》2021年第4期1128-1134,共7页Chinese Traditional and Herbal Drugs

基　　金：国家重点研发计划资助(2019YFC1710603);国家自然科学基金项目(81773910,82074004);江苏高校“青蓝工程”项目(2020)。

摘　　要：目的基于传统煎药工艺建立不同产地黄芪的HPLC指纹图谱,用于黄芪药材质量的评价。方法采用中药色谱指纹图谱相似度评价系统(2004A版)对15批不同产地的黄芪药材的HPLC指纹图谱进行相似度评价,并使用SPSS22.0软件进行聚类分析和主成分分析。结果选取了12个色谱峰作为指纹图谱共有峰,15批样品的相似度计算结果均大于0.990,说明各产地黄芪药材具有较好的一致性;通过聚类分析可将15批样品聚为4类;主成分分析采用3个主成分对黄芪药材进行综合评价,综合得分结果显示,甘肃民乐县所产批号为HQ-18001(S1)、HQ-18002(S2)和甘肃岷县所产批号为HQ-18007(S7)、HQ-18006(S6)的黄芪药材在所有样品中的综合得分位于前4名。结论建立基于传统煎药工艺的黄芪药材质量评价方法操作简单,重复性好,结果可靠,可以用于黄芪药材的质量控制和评价。Objective To establish a HPLC fingerprints evaluation method for the quality evaluation of Astragali Radix based on traditional decoction process taking water as solvent. Methods The fingerprints of 15 batches of Astragali Radix were further evaluated by chemometrics methods. The similarity analyzed with "Similarity Evaluation System for Chromatographic Fingerprint of Chinese Materia Medica 2004 A", and hierarchical clustering analysis(HCA) and principal component analysis(PCA) were performed by SPSS 22.0. Results There were 12 common peaks, and the similarity degrees of 15 batches of samples were more than 0.990, which showed that all the samples from different origins were of good consistency. The samples were divided into four clusters by HCA. The result of PCA showed that the three factors were chosen, the quality of samples could be evaluated basically. According to the composite score, the quality of the batches of HQ-18001(S1) and HQ-18002(S2) of Astragali Radix produced in Minle County of Gansu Province and the batches of HQ-18007(S7) and HQ-18006(S6) produced in Minle County of Gansu Province, were the top four in all the samples. Conclusion The method is simple, reproducible, and reliable, which can be used for quality control and evaluation of Astragali Radix from different origins.

关 键 词：黄芪 HPLC 指纹图谱 质量评价 化学计量学 

分 类 号：R286.2[医药卫生—中药学]