龟嗜皮菌的快速鉴定及其在鳄蜥皮肤病原检测中的应用  

Rapid Identification of Austwickia chelonae and Its Application in Pathogenic Detection of Dermatosis in Crocodile Lizards

作　　者：江海英 陈金平 黄铭威 林立波 李林妙 张秀娟 李官钰 JIANG Hai-Ying;CHEN Jin-Ping;HUANG Ming-Wei;LIN Li-Bo;LI Lin-Miao;ZHANG Xiu-Juan;LI Guan-Yu(Guangdong Key Laboratory of Animal Conservation and Resource Utilization,Guangdong Public Laboratory of Wild Animal Conservation and Utilization,Institute of Zoology,Guangdong Academy of Sciences,Guangzhou 510260,China)

机构地区：[1]广东省科学院动物研究所,广东省动物保护与资源利用重点实验室,广东省野生动物保护与利用公共实验室,广州510260

出　　处：《动物学杂志》2021年第1期111-118,共8页Chinese Journal of Zoology

基　　金：广东省科学院科技发展专项(No.2018GDASCX-0107,2019GDASYL-0105046);广州市科技创新委员会项目(No.201804020080);国家林业和草原局项目(No.2020076079,2019072)。

摘　　要：龟嗜皮菌(Austwickia chelonae)感染爬行类、鸟类、哺乳类等,造成野生动物和家养动物患皮肤病甚至死亡,近年来这种病原体在Ⅰ级保护动物鳄蜥(Shinisaurus crocodilurus)的救护种群中暴发。传统的病原检测方法费时耗力。本文基于龟嗜皮菌全基因组序列开发了特异性高、方便快捷的龟嗜皮菌检测方法,并应用于鳄蜥的皮肤病风险预测。本研究开发的3对检测龟嗜皮菌的特异引物中,AC3引物的使用效果最好。Austwickia chelonae infects reptiles,birds,mammals,etc.,causing skin diseases or deaths in the wild and domestic animals.In recent years,this pathogen outbroke in the rescue population of the first-class protected animal-Crocodile Lizards(Shinisaurus crocodilurus).Traditional methods for pathogen detection are labor-consuming and time-costing.Therefore,this study developed a highly specific and convenient method for the detection of Austwickia chelonae based on its whole genome sequence,and applied this method to the risk prediction of skin disease in crocodile lizards.Among the three pairs of specific primers for A.chelonae detection developed in this paper,primer set AC3 shows the best results.

关 键 词：龟嗜皮菌 鉴定 皮肤病 鳄蜥 

分 类 号：Q95-3[生物学—动物学]