电针对膜迷路积水豚鼠耳蜗积水程度及血管纹细胞超微结构的影响  

Effect of electro-acupuncture on the morphology of cochlea and ultrastructure of stria vascularis cell in guinea pigs with endolymphatic hydrops

作　　者：蒋丽元[1] 史莹莺 叶恬恬 杨俊文 JIANG Liyuan;SHI Yingying;YE Tiantian;YANG Junwen(Department of Acupuncture and Rehabilitation,Hangzhou Hospital of Traditional Chinese Medicine,Hangzhou 310007,China)

机构地区：[1]杭州市中医院针灸康复科,310007 [2]杭州市丁桥医院针灸康复科 [3]浙江中医药大学第一临床医学院

出　　处：《浙江医学》2021年第6期589-592,I0003,共5页Zhejiang Medical Journal

基　　金：国家自然科学基金资助项目(81704153)。

摘　　要：目的观察电针对膜迷路积水模型豚鼠耳蜗积水程度及血管纹细胞超微结构的影响,探讨电针对梅尼埃病膜迷路积水的干预作用及可能的调节机制。方法将健康雄性豚鼠39只按随机数字表法分为电针组、模型组、空白组,每组13只。电针组与模型组采用腹腔注射醋酸去氨加压素建立膜迷路积水模型。电针组豚鼠固定后,取百会、听宫穴,采用100 Hz频率进行电针治疗,留针20 min,1次/d,连续治疗10 d;模型组仅作固定,不予其他处理;空白组不作干预。干预结束后,观察3组豚鼠一般行为学变化,通过HE染色法观察耳蜗蜗管积水程度,并计算蜗管横截面积/(蜗管横截面积+前庭阶横截面积)比值(R值);采用透射电镜观察豚鼠耳蜗血管纹细胞超微结构变化。结果(1)经造模后,电针组和模型组豚鼠普遍出现精神萎靡,轻微脱毛,步态迟缓,进食减少,体重增长缓慢甚至下降,活动减少,耳郭反应迟钝。经电针干预后,电针组豚鼠精神状况及耳郭反应灵敏度与模型组比较均有所改善,但电针组豚鼠皮毛光亮程度、饮食、体重、自发活动情况与模型组比较无差别。(2)3组R值比较差异有统计学意义,模型组高于电针组与空白组(均P<0.01)。(3)透射电镜观察结果显示:模型组豚鼠耳蜗血管纹细胞细胞器受损,出现线粒体肿胀,空泡化变性,线粒体嵴断裂甚至消失;运用电针干预后,电针组豚鼠耳蜗血管纹细胞水肿明显减轻,线粒体空泡化变性及肿胀程度明显改善。结论电针百会、听宫穴能减轻豚鼠膜迷路积水程度,血管纹细胞超微结构病理状态的改善可能是其作用机制之一。Objective To investigate the effect of electro-acupuncture(EA)on the morphology of cochlea and ultrastruc-ture of stria vascularis cell in guinea pigs with endolymphatic hydrops(EH).Methods Thirty-nine healthy male guinea pigs were randomly divided into EA group,model group and blank group,with 13 animals per group.Animals in EA group and model group were injected with Desmopressin Acetate to establish EH model,while no treatment was given to blank group.Guinea in EA group were treated with EA at acupuncture points Baihui and Tinggong with current frequencies of 100Hz once daily for consecutive 10 days.After treatment,the severity of cochlear hydrops was evaluated,the ratio of scala media(SM)area to SM+scala vestibuli(SV)area(R value)was calculated.The ultrastructure changes of stria vascularis cells were assayed.Results After the animal model was induced,guinea pigs developed symptoms of depression,slight hair lost,slow gait,reduced food intake,slow weight gain or even decline,reduced activity,and retarded auricle response.After electro-acupuncture intervention,the mental state and auricle response of guinea pigs were improved compared with the model group,but there was no difference between the EA group and the model group in terms of skin appearance,dietary status,body weight and spontaneous activity.R value in model group increased compared with EA group and blank group(both P<0.01).The stria vascularis cells showed morphological changes such as mitochondrial swelling,mitochondrial vacuolar degeneration,break and disappearance of the mitochondrial cristae in model group.In the EA group,the ultramorphological changes in the stria vascularis cells were relatively milder compared with the model group.Conclusion EA treatment may suppress the development of endolymphatic hydrops,and the alleviate the ultramorphological changes and cellular function of hydropic cochlea.

关 键 词：电针 膜迷路积水 血管纹细胞 超微结构 

分 类 号：R245[医药卫生—针灸推拿学]