消肿止痛合剂对大鼠缺血皮瓣血管再生及VEGF-Dll4/Notch信号通路的影响  

Effect of Xiaozhongzhitong mixture on vegf-dll4/Notchsignaling pathway of ischemic skin flap in rats

作　　者：姚兴璋[1] 刘涛 何志军[1] 李兴勇 宋渊[1] 李岩[1] 李金鹏[1] 陈文[1] 王海刚 何元旭 YAO Xingzhang;LIU Tao;HE Zhijun;LI Xingyun;SONG Yuan;LI Yan;LI Jinpeng;CHEN Wen;WANG Haigang;HE Yuanxu(Department of Orthopedics,Gansu Hospital of Traditional Chinese Medicine,Lanzhou,Gansu 730050,China;Department of Orthopedics,Gansu Provincial People s Hospital,Lanzhou,Gansu 730000,China;Clinical College of Traditional Chinese medicine,Gansu University of Traditional Chinese Medicine,Lanzhou,Gansu 730020,China)

机构地区：[1]甘肃省中医院骨科,甘肃兰州730050 [2]甘肃省人民医院骨科,甘肃兰州730000 [3]甘肃中医药大学中医临床学院,甘肃兰州730020

出　　处：《暨南大学学报（自然科学与医学版）》2021年第2期164-171,共8页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：国家自然科学基金资助项目(81660802;81860863)。

摘　　要：目的:观察消肿止痛合剂对大鼠缺血皮瓣血管再生及VEGF-Dll4/Notch信号通路的影响.方法:将240只SD大鼠随机分为6组,分别为空白组、假手术组、模型组、消肿止痛合剂组(消肿组)、消肿止痛合剂+Notch阻断剂MK-0752组(消肿+MK组)、消肿止痛合剂+VEGF阻断剂Axitinib组(消肿+AXI组).用透明硫酸纸准确描绘各组皮瓣形态,分析皮瓣成活率;用免疫组化法测定皮瓣组织中VEGFA的蛋白表达,Western blot法检测大鼠皮瓣组织中VEGFA、Notch和Dll4蛋白的表达.结果:(1)第5、10、15、20天消肿组皮瓣成活情况显著高于模型组(P<0.05),加入VEGF阻断剂后,消肿+AXI组皮瓣成活率下降(P<0.05),当加入Notch阻断剂后,消肿+MK组皮瓣成活率上升(P<0.05);(2)与模型组相比,消肿止痛合剂干预后,VEGFA、Notch和Dll4蛋白表达量显著升高(P<0.05).与消肿组相比,VEGF阻断剂干预后,VEGFA、Notch和Dll4蛋白表达量降低(P<0.05).Notch阻断剂干预后,VEGFA蛋白表达水平增加,Notch、Dll4蛋白表达增加的趋势被有效抑制(P<0.05).结论:消肿止痛合剂能够促进皮瓣的成活率,可能与影响VEGF-Dll4/Notch信号转导通路有关.Objective:To observe the effect of Xiaozhongzhitong mixture on vascular regeneration and vegf-dll4/Notch signal pathway of random flap in rats.Methods:A total of 240 SD rats were randomly divided into 6 groups:blank group,sham operation group,model group,Xiaozhongzhitong mixture group(Xiaozhong group),Xiaozhongzhitong mixture+notch blocker mk-0752 group(Xiaozhong+MK group),Xiaozhongzhitong mixture group+VEGF blocker axitinib group(Xiaozhong+ax group).Using transparent sulfuric acid paper to accurately depict the shape of each group of flaps and analyze the survival rate of the flaps;using immunohistochemical method to detect the protein expression of VEGFA in the flap tissues,Western blot method to detect the protein expression of VEGFA,notch and Dll4 in the flap tissues of rats.Results:(1)On the 5th,10th,15th and 20th day,the survival rate of flaps in detumescence group was significantly better than that in model group P The protein expression increased significantly(P<0.05).Compared with xiaozhongzhi group,the expression of VEGFA,notch and Dll4 decreased(P<0.05).After the intervention of notch blocker,the expression of VEGFA protein increased,and the expression of notch and Dll4 protein was inhibited(P<0.05).Conclusion:Xiaozhongzhitong mixture can promote the survival rate of flap,which may be related to the influence of vegf-dll4/Notch signal transduction pathway.

关 键 词：消肿止痛合剂 血管再生 VEGF-Dll4/Notch信号通路 大鼠 

分 类 号：R274.3[医药卫生—中医骨伤科学]