UPLC同时测定生脉饮口服液中五味子醇甲、五味子醇乙、五味子甲素的含量  

Simultaneous Determination of Schisandrin A,Schisandrin B and Deoxyschizandrin in Shengmaiyin Oral Liquid by UPLC

作　　者：支旭然[1] 张黎媛[1] 赵娜[1] 董占军[1] ZHI Xuran;ZHANG Liyuan;ZHAO Na;DONG Zhanjun(Hebei General Hospital,Shijiazhuang 050051,China)

机构地区：[1]河北省人民医院,石家庄050051

出　　处：《中国现代应用药学》2021年第4期426-429,共4页Chinese Journal of Modern Applied Pharmacy

基　　金：河北省医学科学研究重点课题计划项目(20170325)。

摘　　要：目的建立UPLC测定生脉饮口服液中有效成分(五味子醇甲、五味子醇乙、五味子甲素)含量的方法。方法采用XBridge?BEH C18色谱柱(2.1 mm×100 mm,2.5μm),以乙腈(A)-水(B)为流动相进行梯度洗脱,流速0.5 mL·min^(–1),进样量5μL;柱温为40℃;检测波长为203nm。结果五味子醇甲、五味子醇乙、五味子甲素检测质量浓度线性范围分别为1.950~124.8μg·mL^(-1)(r=0.9999)、1.375~88.00μg·mL^(-1)(r=0.9999)、1.594~102.0μg·mL^(-1)(r=0.9999);定量限≤0.1375μg·mL^(-1),检测限≤0.0688μg·mL^(-1);仪器精密度、稳定性、重复性试验的RSD≤1.18%;加样回收率分别为98.65%(RSD=0.82%),98.47%(RSD=1.21%),99.24%(RSD=0.99%)。结论该方法操作简便,仪器精密度、稳定性、重复性好,可用于生脉饮口服液中五味子醇甲、五味子醇乙、五味子甲素含量测定。OBJECTIVE To establish quantitative analysis method of effecctive components(schisandrin A,schisandrin B and deoxyschizandrin)in Shengmaiyin oral liquid by UPLC.METHODS The samples were separated on a XBridge?BEH C18(2.1 mm×100 mm,2.5μm).The mobile phase was composed of acetonitrile(A)and water(B)with gradient elution,the flow rate was 0.5 m L·min-1 and the injection volume was 5μL.The column temperature was 40℃and detection wavelength was 203 nm.RESULTS The linear ranges were 1.950-124.8μg·m L^(-1)for schisandrin A(r=0.9999),1.375-88.00μg·m L^(-1)for schisandrin B(r=0.9999),1.594-102.0μg·m L^(-1)for deoxyschizandrin(r=0.9999).The limits of quantify were no more than 0.1375μg·mL^(-1),limits of detection were no more than 0.0688μg·m L^(-1);RSDs of instrument precision,stability and reproducibility tests were≤1.18%;the recoveries were 98.65%(RSD=0.82%),98.47%(RSD=1.21%),99.24%(RSD=0.99%),respectively.CONCLUSION The method is simple,precise,stable and reproducible,and can be used for simultaneous determination of schisandrin A,schisandrin B and deoxyschizandrin in Shengmaiyin oral liquid.

关 键 词：超高效液相色谱法 生脉饮口服液 五味子醇甲 五味子醇乙 五味子甲素 

分 类 号：R917.101[医药卫生—药物分析学]