环境、茎段状态与无糖培养基对葡萄试管苗生根的影响  

Effects of environment,stem section state and sugar-free medium on rooting of grape plantlet in vitro cultured in soil supporting medium

作　　者：董媛媛 柴慈江 夏瑞 黄洁萍 岑芬 Dong Yuanyuan;Chai Cijiang;Xia Rui;Huang Jieping;Cen Fen(College of Horticulture and Landscape,Tianjin Agricultural University,Tianjin 300392,China)

机构地区：[1]天津农学院园艺园林学院,天津300392

出　　处：《天津农学院学报》2021年第1期17-20,33,共5页Journal of Tianjin Agricultural University

基　　金：天津市林果现代农业产业技术体系创新团队项目(ITTFPRS2018002)

摘　　要：本项研究旨在完善葡萄试管苗土壤支撑生根培养技术。分别对使用含糖与否的培养基、接种两种状态的试管苗茎段及两种培养环境进行了试验。结果表明:在不含糖的培养基中试管苗根长与茎长显著高于含糖培养基,茎段枯死率和叶片变色率显著低于含糖培养基;接种新壮茎段试管苗的生根率、根长、茎长及叶数均显著高于老弱茎段;6月份露地环境中培养的试管苗生根率及茎长与培养室环境无显著差异,且生根率达到92.5%。在‘玫瑰香’葡萄试管苗的土壤支撑生根培养中,以使用不含糖培养基和接种新壮茎段为宜,6月份可在露地环境中培养。This paper aimed at perfecting the technique of grape micro-shut rooting in vitro cultured in medium supported by soil.The experiments of using medium containing sucrose or not,inoculating stem section in two states and culturing in two environments have been done respectively.The results showed that the root length and the stem length of the plantlet cultured in medium without sucrose were obviously higher than that in medium containing sucrose,but the stem withered rate and the leaf color changing rate were obviously lower than that in medium containing sucrose.Rooting rate,the root length,the stem length and the leaf number of the younger stem section inoculated were obviously higher than that of the older stem section.With the rooting rate of 92.5%,the rooting rate and the stem length of the plantlet cultured in field environment in June were of no obvious difference compared with that of the plantlet cultured in incubation chamber.In rooting culture in soil supporting medium of‘Muscat Hamburg’grape plantlet in vitro,the medium without sucrose should be used and the younger stem section should be inoculated,and the rooting culture can be in progress in field environment in June.

关 键 词：葡萄 蔗糖 茎段 露地 瓶内生根 

分 类 号：S604.3[农业科学—园艺学] S723.123.6