原产地与入侵地椰子织蛾遗传分化特征  

Genetic and divergence characteristics of Opisina arenosella from native and invasive areas

作　　者：杨帆[1,2] 李优佳[1] SHAMEER K S 阎伟 吕宝乾[5] 蒋方一丁[1] 章雨璐 涂艳 齐可欣 YANG Fan;LI Youjia;SHAMEER K S;YAN Wei;LÜBaoqian;JIANG Fangyiding;ZHANG Yulu;TU Yan;QI Kexin(College of Forestry,Hainan University,Haikou,Hainan 570228,China;Key Laboratory of Genetic and Germplasm Innovation of Tropical Featured Tree and Ornamental Plants,Ministry of Education,Haikou,Hainan 570228,China;Insect Ecology&Ethology Laboratory,Department of Zoology,University of Calicut,Calicut 673635,India;Coconut Research Institute,Chinese Academy of Tropical Agricultural Sciences,Wenchang,Hainan 571339,China;Environment and Plant Protection Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou,Hainan 571101,China)

机构地区：[1]海南大学林学院,海南海口570228 [2]热带特色林木花卉遗传与种质创新教育部重点实验室,海南海口570228 [3]Insect Ecology&Ethology Laboratory,Department of Zoology,University of Calicut,Calicut 673635,India [4]中国热带农业科学院椰子研究所,海南文昌571339 [5]中国热带农业科学院环境与植物保护研究所,海南海口571101

出　　处：《福建农林大学学报（自然科学版）》2021年第2期178-183,共6页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：海南省自然科学基金(318QN195);海南大学科研启动基金项目[KYQD(ZR)1821];农业农村部农业国际交流与合作项目(BARTP-08-LBQ);中国热带农业科学院基本业务费(1630042017011)。

摘　　要：椰子织蛾来源于印度和斯里兰卡,已成功入侵我国华南地区4个省,对我国棕榈植物及城市园林景观造成严重威胁.利用COⅠ、COⅡ和COⅢ3个基因片段对原产地(印度)和入侵地(中国、马来西亚、泰国和印度尼西亚)共12个地区的107份椰子织蛾样品的遗传分化特征进行分析,结果表明:原产地与入侵地种群的核苷酸组成存在34个变异位点,38%为嘌呤转换,62%为嘧啶转换;入侵中国、马来西亚、印度尼西亚和泰国的种群均为同一种基因型.系统发育树表明椰子织蛾存在两个遗传支系,即原产地支系与入侵地支系,遗传距离为1.3%,说明椰子织蛾种群已产生遗传分化.Opisina arenosella,originating from India and Sri Lanka,has successfully invaded South China and poses tremendous threat to palm plantation and urban landscape in China.In this study,COⅠ,COⅡand COⅢgene fragments were used to analyze the genetic and divergence characteristics of O.arenosella samples collected from 12 regions,including the place of origin(India)and 4 invasive regions(China,Malaysia,Thailand and Indonesia).The results showed that there were 34 variation sites between the populations from the native and the invasive sites,including 38%purine conversion and 62%pyrimidine conversion.Phylogenet-ic trees showed that there were 2 genetic branches of the coconut moth,namely the origin branch and invasive branch,with a genetic distance of 1.3%,suggesting that the genetic divergence had developed.

关 键 词：椰子织蛾 线粒体DNA 遗传分化 变异位点 

分 类 号：S763.42[农业科学—森林保护学]