外源水杨酸和甜菜碱对NaCl胁迫下茶花凤仙种子萌发的影响  

Effects of Exogenous SA and GB on Seed Germination of Impatiens balsamena Under NaCl Stress

作　　者：姜云天 曲广男 国怡 潘立雪 李玉梅[3] JIANG Yuntian;QU Guangnan;GUO Yi;PAN Lixue;LI Yumei(College of Life Science,Tonghua Normal University,Tonghua,Jilin 134002;College of Life Science,Yunnan University,Kunming,Yunnan 650500;Institute of Ecological Environment,Jilin Normal University,Siping,Jilin 136000)

机构地区：[1]通化师范学院生命科学学院,吉林通化134002 [2]云南大学生命科学学院,云南昆明650500 [3]吉林师范大学生态环境研究所,吉林四平136000

出　　处：《北方园艺》2021年第5期77-83,共7页Northern Horticulture

基　　金：吉林省科技发展计划资助项目(20190304012YY);吉林省大学生创新创业训练计划资助项目(201910202047);通化师范学院学生创新与实践技能训练资助项目(cs2019041)。

摘　　要：以茶花凤仙(Impatiens balsamena)种子为试材,采用双层滤纸法,研究了100 mmol·L^(-1)NaCl胁迫条件下,外源SA(0.5、1.0、1.5、2.0 mmol·L^(-1))和GB(0.4、0.8、1.2、1.6 mmol·L^(-1))浸种预处理对茶花凤仙种子萌发及幼苗生长的影响,以期明确茶花凤仙种子萌发期2种外源物质对NaCl胁迫的缓解效应,降低NaCl胁迫对茶花凤仙的伤害。结果表明:NaCl胁迫显著抑制了茶花凤仙种子的萌发,发芽率、发芽势、发芽指数、活力指数、胚芽长、胚根长和苗鲜质量均显著下降,平均发芽时间显著延长;1.5 mmol·L^(-1)SA和0.8、1.2 mmol·L^(-1)GB对NaCl胁迫下茶花凤仙种子萌发和幼苗生长具有明显促进作用,而SA和GB浓度过高或过低则对NaCl胁迫的缓解效应下降,甚至加重盐害。利用隶属函数综合评价得出,100 mmol·L^(-1)NaCl胁迫下,1.5 mmol·L^(-1)SA和0.8 mmol·L^(-1)GB对茶花凤仙种子萌发及幼苗生长的综合调控效果较好,且0.8 mmol·L^(-1)GB对NaCl胁迫的缓解效果优于1.5 mmol·L^(-1)SA。To define the role of exogenous salicylic acid and exogenous glycine betaine in alleviating the damage of NaCl stress on Impatiens balsamena during the germination period, the effects of two exogenous substances on the seed germination and seedling growth of Impatiens balsamena under NaCl stress were investigated.The seeds of Impatiens balsamena after presoaking treatments with different concentrations of SA(0.5,1.0,1.5,2.0 mmol·L^(-1)) and GB(0.4,0.8,1.2,1.6 mmol·L^(-1)) were cultured adopting a double-layer filter paper method under 100 mmol·L^(-1) NaCl solution.The results showed that germination rate, germination potential, germination index, vigor index, embryo length, radicle length and fresh weight of seedlings reduced significantly under NaCl stress, and mean germination time was significantly prolonged.The seed germination and seedling growth of Impatiens balsamena were promoted when SA was 1.5 mmol·L^(-1) and GB were 0.8,1.2 mmol·L^(-1),while the alleviation effect of NaCl stress reduced, and even aggravated the salt injury when SA and GB concentrations were too high or too low.The comprehensive evaluation using membership function revealed that 1.5 mmol·L^(-1)SA and 0.8 mmol·L^(-1)GB had the satisfactory regulatory effects on seed germination and seedling growth of Impatiens balsamena under NaCl stress, and the effect of 0.8 mmol·L^(-1) GB was better than 1.5 mmol·L^(-1) SA.

关 键 词：茶花凤仙 NACL胁迫 水杨酸 甜菜碱 种子萌发 

分 类 号：S681.1[农业科学—观赏园艺]