培补二天膏对老年自发性高血压大鼠血管形态及一氧化氮、血管紧张素Ⅱ的影响  

Effects of Peibuertian Oral Extract on Vascular Morphology,NO and AngⅡin Aged Spontaneously Hypertensive rats

作　　者：张晖[1] 戴斌[1] 吴瑛滢 陈竞纬[1] ZHANG Hui;DAI Bin;WU Ying-Ying;CHEN Jing-Wei(Suzhou Hospital of Traditional Chinese Medicine Affiliated to Nanjing University of Chinese Medicine,Suzhou 215009 Jiangsu,China)

机构地区：[1]南京中医药大学附属苏州市中医医院,江苏苏州215009

出　　处：《广州中医药大学学报》2021年第3期570-575,共6页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：江苏省333工程项目(编号:LGY2019012);第五批姑苏卫生人才培养项目(编号:GSWS2019022);苏州市科技局项目(编号:SS201745)。

摘　　要：【目的】观察培补二天膏对老年自发性高血压大鼠(SHR)血压及血管的影响。【方法】将50只SHR大鼠随机分为模型组,西药对照组,膏方低、中、高剂量组,每组10只;另将10只Wistar京都大鼠(WKY)作为正常对照组。膏方低、中、高剂量组给予培补二天膏混悬液(对应剂量分别为0.58、1.15、3.46 g·kg^(-1)·d^(-1))灌胃;西药对照组给予厄贝沙坦混悬液(剂量为10.13 mg·kg^(-1)·d^(-1))灌胃;模型组与正常对照组给予同体积蒸馏水灌胃。共4周,期间每周监测收缩压(SBP)。给药结束后,测血清一氧化氮(NO)、血管紧张素Ⅱ(AngⅡ)含量,苏木素-伊红染色(HE)观察胸主动脉组织病理改变。【结果】(1)实验过程中,模型组SBP始终高于正常对照组(P<0.01);膏方各剂量组及西药对照组干预1~4周后均能降低SBP;与模型组比较,膏方中剂量组治疗4周降压明显(P<0.01),膏方高剂量组与西药对照组治疗1周即出现明显的降压效果(P<0.05)。(2)与正常对照组比较,模型组血清NO含量明显下降,AngⅡ含量明显升高(均P<0.05);与模型组比较,膏方高剂量组NO含量明显升高,AngⅡ含量明显降低(均P<0.05),西药对照组AngⅡ含量降低明显(P<0.05)。(3)胸主动脉组织病理HE染色结果显示:SHR大鼠胸主动脉形态学明显改变;膏方各剂量组及西药对照组均可改善SHR大鼠胸主动脉形态学变化。【结论】培补二天膏可有效降压及保护血管,其作用可能是通过调节血液中AngⅡ和NO的水平来实现。Objective To observe the effects of Peibuertian Oral Extract on blood pressure and blood vessels in aged spontaneously hypertensive rats(SHR).Methods Fifty SHR rats were randomly divided into model group,western medicine control group,low-,middle-and high-dose Oral Extract groups,10 rats in each group,and 10 Wistar Kyoto rats(WKY)were taken as normal control group.The low-,middle-and high-dose Oral Extract groups were given intragastric administration of Peibuertian Oral Extract suspension(at the corresponding dosage of 0.58,1.15,3.46 g·kg^(-1)·d^(-1),respectively),the western medicine control group was given intragastric administration of irbesartan suspension(at the dosage of 10.13 mg·kg^(-1)·d^(-1)),and the model group and normal control group were given intragastric administration of the same volume of distilled water.The treatment lasted 4 weeks,during the period blood pressure(SBP)was monitored weekly.At the end of the experiment,the serum nitric oxide(NO)and angiotensinⅡ(AngⅡ)contents were measured,and the pathological changes of thoracic aorta were observed by hematoxylin eosin staining(HE).Results(1)During the experiment,SBP in the model group was always higher than that in the normal control group(P<0.01);the SBP in various dose Oral Extract groups and western medicine control group was reduced after intervention;compared with the model group,the blood pressure in the middle-dose Oral Extract group was significantly decreased for 4 weeks of treatment(P<0.01),and the high-dose group and western medicine control group had obvious antihypertensive effect for one week of treatment(P<0.05).(2)Compared with the normal control group,the serum NO content in the model group was significantly decreased,and AngⅡcontent was significantly increased(all P<0.05);compared with the model group,the NO level was significantly increased,and the AngⅡlevel was significantly decreased in the high-dose Oral Extract group(all P<0.05),and AngⅡlevel was significantly decreased in the western medicine control group(P

关 键 词：培补二天膏 高血压 自发性高血压大鼠 血管保护 一氧化氮 血管紧张素Ⅱ 大鼠 

分 类 号：R285.5[医药卫生—中药学]