基于Raman和FTIR揭示避雨栽培甜樱桃叶片光合色素变化的光谱学分析  

Spectral Analysis of Changes in Photosynthetic Pigment Composition in Leaves of Sweet Cherry Tree Under Rain-Shelter Cultivation Based onRaman and FTIR

作　　者：张惠敏 侯黔东 吴亚维[3] 屠凯 李权[4] 文晓鹏[1,2] ZHANG Hui-min;HOU Qian-dong;WU Ya-wei;TU Kai;LI Quan;WEN Xiao-peng(College of Forest/Institute for Forest Resources&Environment of Guizhou,Guizhou University,Guiyang 550025,China;Institute of Agro-bioengineering/The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education),Guizhou University,Guiyang 550025,China;Institute of Fruit Tree Research,Guizhou Academy of Agricultural Sciences,Guiyang 550006,China;Kaili University,Kaili 556011,China)

机构地区：[1]贵州大学林学院/贵州省森林资源与环境研究中心,贵州贵阳550025 [2]贵州大学农业生物工程研究院/山地植物资源保护与种质创新教育部重点实验室,贵州贵阳550025 [3]贵州省农业科学院果树科学研究所,贵州贵阳550006 [4]凯里学院,贵州凯里556011

出　　处：《光谱学与光谱分析》2021年第4期1171-1176,共6页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(31760191);贵州省研究生科研基金项目(黔教合YJSCXJH(2019)104);贵州省农业攻关计划项目(黔科合NY[2015]3002-2号)资助。

摘　　要：为提高甜樱桃果实的品质和产量,中国南方地区普遍采用了避雨栽培甜樱桃果树来避免其坐果率低、落果以及果实畸形等问题,但同时也导致了其光合作用受到影响。植物或藻类中的叶绿素和类胡萝卜素等光合色素在光收集和介导对各种内源性刺激的应激反应中起着不可替代的作用。为便捷快速检测果树光合作用中叶片的光合色素变化,采用拉曼光谱(Raman spectra)和傅里叶红外光谱(FTIR)对避雨和露地栽培的甜樱桃叶片进行了研究。经测定和分析甜樱桃叶片200~3500 cm^(-1)范围的Raman光谱,对400~800,800~1250和1250~1650 cm^(-1)三个波数段特征峰值的标定和指认,结果表明:甜樱桃叶片对拉曼散射较敏感区主要在500~1700 cm^(-1)波段内。960~1800 cm^(-1)范围类胡萝卜素(番茄红素、β-胡萝卜素和叶黄素)的Raman光谱包含4条主要峰,分别为1526,1157,1005和960 cm^(-1);露地栽培的甜樱桃叶片Raman强度明显低于避雨栽培。1157和1526 cm^(-1)同样还是叶绿素的Raman光谱特征峰,总体分析表明露地比避雨栽培甜樱桃叶片中的光合色素含量更低。1157,1520和1526 cm^(-1)特征谱线对应C—C单键和C C双键的对称伸缩振动,其相对强度可以作为甜樱桃叶片内叶绿素、类胡萝卜素等光合色素以及纤维素含量的判断依据。FTIR表征叶绿素的振动峰位振动强度弱,振动耦合复杂,难以指认。通过对甜樱桃叶片化学组分FTIR光谱图进行二阶导数求导处理凸显了峰的位置,提高了图谱的分辨率。峰位在1437和1551 cm^(-1)的β-胡萝卜素的特征峰明显,露地栽培相比避雨栽培甜樱桃叶片这两个特征峰的吸光度偏小,表明露地栽培甜樱桃叶片中的β-胡萝卜素含量比避雨栽培要少。该研究为不同栽培模式下植物叶片光合色素的光谱学研究提供了参考。To improve the of quality and yield of sweet cherry fruits,sweet cherry trees were cultivated under rain-shelter to avoid the problems of low fruit setting rate,falling fruit and fruit malformation in southern China.Sweet cherry trees under rain-shelter cultivation had an obvious negative effect on photosynthesis.In both plants and algae,photosynthetic pigments such as chlorophylls and carotenoids play irreplaceable roles in light harvesting and mediating stress responses to a variety of endogenous stimuli.This research aimed to detect changes of photosynthetic pigments in leaves that affect photosynthesis of fruit trees quickly and conveniently.The experiment took sweet cherry leaves in two different cultivation pattern,open-field and rain-shelter cultivation,as the research objects,and determined its Raman spectrum in the range of 200~3500 cm^(-1).The analysis is performed,and the characteristic peaks are calibrated and designated from three wave number bands of 400~800,800~1250 and 1250~1650 cm^(-1).According to the Raman spectrum characteristic value,it is concluded that the sweet cherry leaves have a relatively small Raman scattering.Sensitivity is mainly concentrated in the 500~1700cm-1 band.The analysis of Raman spectrum in the range of 960~1800 cm^(-1)found that characterizes the carotenoids(lycopene,β-carotene and lutein)mainly contains 4 main peaks,which are 1526,1157,1005 and 960 cm^(-1),the Raman intensity of leaves of sweet cherry tree under open-field cultivation is significantly lower than that of rain-shelter cultivation.1157 and 1526 cm^(-1)are also the Raman spectrum characteristic peaks of chlorophyll.Overall analysis shows that photosynthetic pigment content in leaves of sweet cherry under open-field cultivation is lower than that of under rain-shelter cultivation.The characteristic spectral lines of 1157,1520 and 1526 cm^(-1)correspond to symmetrical stretching vibrations of C—C single bond and C C double bond,and their relative strengths can be used as the basis for judging the content of

关 键 词：类胡萝卜素 叶绿素 甜樱桃 特征峰 

分 类 号：S66-3[农业科学—果树学]