天麻素干预脊髓损伤模型兔运动功能及神经生长相关蛋白43的表达  

Gastrodin interferes with motor function recovery and growth associated protein-43 expression in a rabbit model of spinal cord injury

作　　者：张衡 张贤平 戴厚杰 黄昌钊 王锐英[1] Zhang Heng;Zhang Xianping;Dai Houjie;Huang Changzhao;Wang Ruiying(Department of Traumatic Surgery for Limbs,the Affiliated Hospital of Guilin Medical University,Guilin 541001,Guangxi Zhuang Autonomous Region,China)

机构地区：[1]桂林医学院附属医院四肢创伤外科,广西壮族自治区桂林市541001

出　　处：《中国组织工程研究》2021年第29期4626-4631,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金资助项目(81460198),项目负责人:王锐英。

摘　　要：背景:研究报道,天麻素在脊髓损伤中具有抗炎、抗氧化及神经保护作用。目的:研究天麻素对动物脊髓损伤中运动功能的恢复及神经生长相关蛋白43表达的影响。方法:将30只新西兰大白兔随机分为假手术组、对照组、实验组,每组10只。对照组、实验组应用改良Allen’s打击法建立脊髓损伤模型,实验组静脉注射天麻素100 mg/(kg•d),假手术组和对照组各静脉注射10 mL生理盐水,连续给药7 d。造模后1,3,5,7 d,对各组大鼠进行神经功能评分和神经电生理检测;造模7 d后取损伤部位脊髓组织,进行苏木精-伊红染色、尼氏染色、Western blot检测、免疫荧光观察。实验方案经桂林医学院动物实验伦理委员会批准(批准编号为GMC201805018)。结果与结论:①实验组造模后不同时间点的后肢神经功能评分明显高于对照组(P<0.05);②与假手术组比较,对照组造模后不同时间点的运动诱发电位发作潜伏期变长(P<0.05),峰间振幅降低(P<0.05);与对照组比较,实验组造模后3,5,7 d的运动诱发电位发作潜伏期缩短(P<0.05),峰间振幅升高(P<0.05);③苏木精-伊红染色显示,对照组可见脊髓空洞,结构不完整,灰质与白质界限不清,灰质中神经元坏死凋亡;实验组可见空洞坏死,脊髓组织结构完整性好,灰质结较为清晰;④尼氏染色显示,对照组无神经元,可见大量胶质细胞增生;实验组可见神经元存活,胶质细胞增生相对较少;⑤Western blot检测与免疫荧光观察显示,对照组与实验组的神经生长相关蛋白43表达高于假手术组,实验组明显高于对照组;⑥结果表明,天麻素能够促进脊髓损伤兔运动功能的恢复,并且可能通过增加神经生长相关蛋白43的表达来促进脊髓神经轴突的再生。BACKGROUND:Studies have reported that gastrodin has anti-inflammatory,antioxidant and neuroprotective effects in the treatment of spinal cord injury.OBJECTIVE:To study the effect of gastrodin on the recovery of motor function and the expression of growth associated protein-43(GAP-43)in animals with spinal cord injury.METHODS:Thirty New Zealand white rabbits were divided into sham operation group,control group and experimental group,with 10 rabbits in each group.The control and experimental groups used modified Allen’s percussion method to establish a spinal cord injury model.The experimental group was injected with 100 mg/(kg•d)gastrodin intravenously.The sham operation and control groups were injected with 10 mL of normal saline intravenously for 7 continuous days.At 1,3,5,and 7 days after modeling,the rats in each group were scored for neurological function and neuroelectrophysiological testing;at 7 days after modeling,the injured spinal cord tissue was taken and stained with hematoxylin-eosin and Nissl,and used for western blot detection,immunofluorescence observation.The experimental protocol was approved by the Animal Experiment Ethics Committee of Guilin Medical University,with an approval No.GMC201805018.RESULTS AND CONCLUSION:The neurological function scores of the hind limbs were significantly higher in the experimental group than the control group at different time points after modeling(P<0.05).Compared with the sham operation group,the latency period of motor evoked potential seizures at different time points after modeling in the control group became longer(P<0.05),and the peak-to-peak amplitude decreased(P<0.05).Compared with the control group,in the experimental group,the motor evoked potential latency was shortened(P<0.05),and the peak-to-peak amplitude increased(P<0.05)at 3,5,and 7 days after modeling.Hematoxylin-eosin staining showed that in the control group,syringomyelia was seen,the structure was incomplete,the boundary between gray matter and white matter was unclear,and neurons in the gr

关 键 词：天麻素 脊髓损伤 神经损伤与修复 神经生长相关蛋白43 神经功能 实验动物 

分 类 号：R459.9[医药卫生—治疗学] R313[医药卫生—临床医学]