应用于dPCR的大视场荧光显微检测系统的设计  

Design of fluorescence microscopy detection system with wide field of view for dPCR

作　　者：王子程 郑继红[1,2] 万新军[1] 孙刘杰[2,3] 陈诚 WANG Zicheng;ZHENG Jihong;WAN Xinjun;SUN Liujie;CHEN Cheng(School of Optical-Electrical and Computer Engineering,University of Shanghai for Science and Technology,Shanghai 200093,China;Shanghai Key Lab of Modern Optical System,University of Shanghai for Science and Technology,Shanghai 200093,China;College of Communication and Art Design,University of Shanghai for Science and Technology,Shanghai 200093,China)

机构地区：[1]上海理工大学光电信息与计算机学院,上海200093 [2]上海理工大学上海市现代光学系统重点实验室,上海200093 [3]上海理工大学出版印刷与艺术设计学院,上海200093

出　　处：《光学技术》2021年第1期1-5,22,共6页Optical Technique

基　　金：上海市科学技术委员会地方院校能力建设项目——高通量PCR基因芯片荧光检测系统研制(18060502500)。

摘　　要：数字聚合酶链式反应(dPCR)作为一种新的核酸检测技术,在生物医学等领域得到广泛的应用。为了进一步提升现有的dPCR检测系统的检测效率,设计了一套基于大视场荧光显微镜的dPCR检测系统,将照明系统与滤波模组设计于显微镜物空间,结合复眼照明系统实现大面积匀光照明,照明面积可达到32mm×22mm,照明均匀性达到80%以上,提出一种结合神经网络的培养皿计数检测方法,可以有效筛选出培养皿中已经蒸发的培养皿。整个系统从相机曝光到检测结束仅需要几秒钟时间,相比传统的图像拼接式检测方法,其检测效率得到了明显提高。As a new nucleic acid detection technology,dPCR has been widely used in biomedicine and other fields.In order to further improve the detection efficiency of the existing dPCR detection system,a set of dPCR detection system based on large field of view fluorescence microscope is designed.The illumination system and filter module are designed in the microscope object space,and combines the compound eye illumination system to achieve a large area uniform illumination,the illumination area can reach 32mm×22mm,and the illumination uniformity can reach more than 80%.A method for counting and detecting petri dishes combined with neural network is proposed,which can effectively pick out the petri dishes that have evaporated in the petri dishes.The system only needs a few seconds from the camera exposure to the end of detection.Compared with traditional image stitching detection methods,the detection efficiency is improved significantly.

关 键 词：数字PCR 照明设计 荧光显微镜 图像处理 

分 类 号：TH742[机械工程—光学工程]