低能量激光对大鼠背根神经节持续受压后痛觉敏感及TRPV4表达的影响  

The effect of low-level laser on the pain sensitivity and the expression of TRPV4 after chronic compression of the dorsal root ganglion in rats

作　　者：洪艺根 高文双 贾磊[1] 魏慧[1] 曲玉娟[1] 岳寿伟[1] HONG Yigen;GAO Wenshuang;JIA Lei(The Rehabilitation Department of Qilu Hospital of Shandong University,Jinan,250012)

机构地区：[1]山东大学齐鲁医院康复科,济南250012

出　　处：《中国康复医学杂志》2021年第2期129-134,共6页Chinese Journal of Rehabilitation Medicine

基　　金：国家自然科学基金面上项目(81772436、81972155)。

摘　　要：目的:观察低能量激光对大鼠背根神经节持续受压(chronic compression of the dorsal root ganglion,CCD)后痛觉敏感及脊髓背角内TRPV4表达及分布的影响。方法:选取健康雄性Wistar大鼠36只,分为空白组、CCD组、激光组各12只,各组均给予常规饲养。激光组在CCD后第4天经皮肤给予L4/L5脊髓节段处810nm低能量激光治疗7天,输出功率150mw,10min/次。并于术前,术后第4、7、11天,分别测量各组大鼠机械刺激缩爪反应阈值和热缩爪潜伏期,观察机械痛阈和热痛阈的变化。同时,利用Western Blot检测各组术后11天TRPV4在脊髓背角中蛋白表达的变化,利用免疫荧光技术检测各组术后11天TRPV4阳性细胞分布。结果:与空白组相比,CCD组在术后第4、7、11天,机械痛阈和热痛阈均降低(P<0.01),与CCD组相比,激光组在术后第7、11天时,机械痛阈和热痛阈明显增高(P<0.01)。与空白组相比较,CCD组大鼠脊髓背角中TRPV4表达显著上调(P<0.01),TRPV4阳性细胞数目升高;给予低能量激光治疗后,激光组脊髓背角TRPV4的蛋白表达较CCD组显著下调(P<0.01),TRPV4阳性细胞数目低于CCD组(P<0.01)。结论:低能量激光治疗可降低CCD后大鼠脊髓背角中TRPV4蛋白表达,减少阳性细胞数目,影响TRPV4对神经病理性疼痛的中枢敏化的作用,缓解CCD后所致的痛觉敏感。Objective:To observe the effect of low-level laser on the pain sensitivity and the expression and distribution of TRPV4 in the dorsal horn of the spinal cord after continuous compression of the dorsal root ganglia(CCD)in rats.Method:Thirty-six healthy male Wistar rats were divided into three groups,blank group,CCD group and laser group.In the laser group,810 nm low-level laser,output power 150 mW,10 min/time,was given to L4/L5 spinal cord segment through skin on the 4 th day after CCD for 7 days.Before and on the 4 th,7 th and 11 th day after operation,the mechanical withdrawal threshold and the thermal withdrawal latency were measured.On the 11 th day after operation,Western Blot was used to detect the protein expression of TRPV4 in spinal dorsal horn,and the distribution of TRPV4 positive cells was detected by immunofluorescence technique.Result:Compared with the blank group,the mechanical withdrawal threshold and the thermal withdrawal latency in the CCD group decreased on the 4 th day significantly(P<0.01),with no changes through 7 th to 11 th day after operation.The mechanical withdrawal threshold and the thermal withdrawal latency in the laser group decreased as the same as in CCD group on the 4 th day significantly(P<0.01).They increased gradually and significantly on the 7 th and 11 th day(P<0.01),but still less than before operation(P<0.01).Compared with the blank group,the expression of TRPV4 in the dorsal horn of the spinal cord and the number of TRPV4 positive cells in the CCD group was significantly up-regulated(P<0.01),but they are nearly the same with the blank group in the laser group.Conclusion:Low-level laser treatment can reduce the expression of TRPV4 protein and reduce the number of TRPV4 positive cells in spinal dorsal horn of rats after CCD,reduce the number of TRPV4 positive cells,so as to lower the central sensitization of TRPV4 to neuropathic pain,and alleviate the pain sensitivity caused by CCD.

关 键 词：低能量激光 背根神经节持续受压 脊髓背角 TRPV4 

分 类 号：R454.2[医药卫生—治疗学] R493[医药卫生—临床医学]