富硒大豆低聚肽的制备及其抗疲劳功能的研究  

Preparation and Anti-Fatigue Function of Selenium-Rich Soybean Oligopeptide

作　　者：白海军[1] Bai Haijun(Heilongjiang Bayi Agricultural University,Daqing 163319)

机构地区：[1]黑龙江八一农垦大学,大庆163319

出　　处：《中国粮油学报》2021年第3期46-50,58,共6页Journal of the Chinese Cereals and Oils Association

摘　　要：为探究富硒大豆低聚肽对抗运动性疲劳的影响,实验以富硒大豆为原料制备大豆蛋白,并以超滤萃取辅助中性蛋白酶和风味蛋白酶双酶酶解法制取富硒大豆低聚肽,并通过ICP-MS对硒含量进行鉴定,最后通过小鼠运动性疲劳实验综合评估富硒大豆低聚肽的抗疲劳活性。结果显示:富硒大豆低聚肽的总硒含量为(90.03±3.23)μg/kg,主要形态为硒代蛋氨酸、硒代半胱氨酸。在运动性疲劳小鼠模型下,四组剂量组均能够延长小鼠负重力竭游泳时长,其中富硒大豆低聚肽高剂量组时间最长约为(37.47±1.33)min,同时,血清生化指标显示小鼠的SOD酶、GSH-Px酶活性相较于模型组显著提高(P<0.05),MDA含量显著降低,肌糖原与肝糖原含量提高。富硒大豆低聚肽对小鼠抗运动性疲劳具有积极作用,在100 mg/(kgbw·d)剂量组下抗疲劳效果显著。To study the effects of selenium-rich soybean oligopeptideonanti-exercise fatigue,the study used the selenium-rich soybean as the raw material to prepare soybean protein.After that,ultrafiltration extractionwas used to assist enzymolysis approach of neutral proteinase and flavourzyme to prepareselenium-rich soybean oligopeptide.Then,the content of selenium was identified by ICP-MS,and the antifatigue activity of selenium-rich soybean oligopeptides was evaluated by the exercise-induced fatigue test in mice.The results indicatedthat the total selenium content of Se rich soybean oligopeptides were(90.03±3.23)μg/kg,and themain forms ofselenium-richsoybean were selenomethionine and selenocysteine.Underthe model of exercise-induced fatigue mice,the four dose groups could prolong the loaded-swimming time of mice.Among the four groups,the selenium-rich soybean group showed a longer swimming time with(37.47±1.33)min.Meanwhile,Serum biochemical index indicated that the activities of SOD and GSH-Px in mice were significantly higher than thosein model group.The content of MDA decreased significantly(P<0.05),and the content of muscle glycogen and liver glycogen was higher.In conclusion,selenium-rich soybean oligopeptide had a positive effect on the antifatigue of mice,and the anti-exercise fatigue effect of 100 mg/(kgbw·d)group was significant.

关 键 词：富硒大豆低聚肽 超滤 抗运动性疲劳 

分 类 号：TS214.2[轻工技术与工程—粮食、油脂及植物蛋白工程]