富血小板血浆对人慢性难愈创面肉芽组织成纤维细胞体外增殖和迁移的影响  

Effects of platelet-rich plasma on in vitro proliferation and migration of fibroblasts from human chronic refractory wound granulation tissue

作　　者：张铁凝 李全[2] 巴特[2] 邵天喜 李芳[2] 王凌峰[2] ZHANG Tie-ning;LI Quan;BA Te;SHAO Tian-xi;LI Fang;WANG Ling-feng(Inner Mongolia University of Science and Technology Baotou Medical College,Baotou 014060,China;Department of Burns,Baogang Hospital of Inner Mongolia,Baotou 014010,China)

机构地区：[1]内蒙古科技大学包头医学院,包头市014060 [2]内蒙古包钢医院烧伤外科,包头市014010

出　　处：《广西医学》2021年第2期205-208,共4页Guangxi Medical Journal

基　　金：内蒙古自治区自然科学基金(2017MS0877);内蒙古自治区包头市科技计划(2017S2001-1-03);内蒙古自治区卫生计生科研计划(201703157)。

摘　　要：目的观察富血小板血浆(PRP)对人慢性难愈创面肉芽组织成纤维细胞体外增殖和迁移的影响。方法取人慢性难愈创面肉芽组织,从中分离成纤维细胞并鉴定。将获得的成纤维细胞分为胎牛血清组、水凝胶组和PRP组,分别采用含有胎牛血清、水凝胶和PRP的培养液培养,观察成纤维细胞生长情况,采用细胞划痕实验观察成纤维细胞的迁移情况。结果培养第5天起,PRP组成纤维细胞数量多于胎牛血清组和水凝胶组,划痕实验第48 h、72 h,PRP组成纤维细胞划痕迁移面积均大于胎牛血清组(均P<0.05)。结论与胎牛血清相比,采用PRP培养人成纤维细胞能更有效地促进成纤维细胞的增殖和迁移。Objective To observe the effects of platelet-rich plasma(PRP)on in vitro proliferation and migration of fibroblasts from human chronic refractory wound granulation tissue.Methods Fibroblasts were separated from human chronic refractory wound granulation tissue and then were identified.The obtained fibroblasts were divided into fetal bovine serum(FBS)group,hydrogel group and PRP group,and the three groups were cultured with culture medium containing FBS,hydrogel and PRP,respectively,to observe the growth of fibroblasts.Wound scratch assay was used to observe the migration of fibroblasts.Results The PRP group had more fibroblasts than the FBS group and the hydrogel group since day 5 of culture,and exhibited greater fibroblasts scratch migration area than the FBS group at the 48th and 72nd hour after wound scratch assay(all P<0.05).Conclusion Compared with FBS,human fibroblasts culture using PRP can more effectively promote the proliferation and migration of fibroblasts.

关 键 词：成纤维细胞 慢性难愈创面 肉芽组织 富血小板血浆 细胞增殖 细胞迁移 体外 

分 类 号：R34[医药卫生—基础医学] R622.1