拔伸松动手法对兔膝关节骨性关节炎关节液中自由基代谢的影响  

Effect of Pulling-Stretching-Loosening Manipulation on Free Radical Metabolism in Synovial Fluid of Rabbit Knee Osteoarthritis

作　　者：王春林 田启东 胡鸾 向勇 艾健 赵志勇 董有康 WANG Chun-lin;TIAN Qi-dong;HU Luan;XIANG Yong;AI Jian;ZHAO Zhi-yong;DONG You-kang(Yunnan University of Traditional Chinese Medicine,Kunming 650500,China;The First Affiliated Hospital of Yunnan University of Traditional Chinese Medicine,Kunming 650021,China)

机构地区：[1]云南中医药大学,云南昆明650500 [2]云南中医药大学第一附属医院,云南昆明650021

出　　处：《云南中医中药杂志》2021年第3期69-72,共4页Yunnan Journal of Traditional Chinese Medicine and Materia Medica

基　　金：国家自然科学基金地区基金项目(81660824);云南省科技计划项目(2014FZ099)。

摘　　要：目的研究拔伸松动手法对兔膝关节骨性关节炎(KOA)模型兔关节液中的超氧化物歧化酶(SOD)、过氧化脂质(LPO)、丙二醛(MDA)的影响,探讨拔伸松动法促进兔KOA软骨损伤修复的机制。方法将80只健康成年家兔随机分为实验组、对照组、模型组、空白组,每组20只。除空白组外,其余各组采用Videman T法建立KOA模型。实验组采用拔伸松动手法干预;对照组塞来昔布胶囊经胃灌药干预;模型组和空白组,不做治疗干预。实验完成后,抽取实验兔膝关节液,黄嘌呤酶法测定超氧化物歧化酶(SOD)活性,硫代巴比妥酸法检测丙二醛(MDA)含量,ELISA法检测过氧化脂质(LPO)含量。结果实验组关节液中SOD浓度高于对照组和模型组(P<0.05);LPO浓度和MDA浓度均低于对照组和模型组(P<0.05)。结论拔伸松动手法可以升高KOA模型兔关节液中SOD浓度,降低LPO和MDA浓度,增强机体清除氧自由基的能力,促进损伤软骨的修复。Objective:To study the effect of pulling-stretching-loosening manipulation on superoxide dismutase(SOD),lipid peroxide(LPO)and malondialdehyde(MDA)in rabbit synovial fluid of rabbit knee osteoarthritis(KOA)model and to explore the mechanism of pulling-stretching-loosening method to promote the repair of rabbit KOA cartilage damage.Methods:80 healthy adult rabbits were randomly divided into experimental group,control group,model group,and blank group,20 rabbits per group.Except for the blank group,the other groups were given Videman T method to establish KOA models.The experimental group was intervened by pulling-stretching-loosening manipulation,the control group was intervened by intragastric administration of Celecoxib capsules and the model group and the blank group were not treated with therapeutic intervention.After the experiment was completed,the experimental rabbit knee joint fluid was extracted.The xanthine enzyme method was used to determine the superoxide dismutase(SOD)activity,the thiobarbituric acid method was used to detect the content of malondialdehyde(MDA),and the ELISA method was used to detect lipid peroxide(LPO)content.Results:The concentration of SOD in the joint fluid of the experimental group was higher than that of the control group and the model group(P<0.05).The concentration of LPO and MDA of the experiment group were lower than that of the control group and the model group(P<0.05).Conclusion:The pulling-stretching-loosening manipulation can increase the SOD concentration in the synovial fluid of KOA model rabbits,reduce the concentration of LPO and MDA,enhance the body’s ability to scavenge oxygen free radicals and promote the repair of damaged cartilage.

关 键 词：拔伸松动手法 关节骨性关节炎 SOD LPO MDA 

分 类 号：R684.3[医药卫生—骨科学]