黄芪葛根配伍对糖尿病大鼠脂肪组织AMPK信号通路的影响  

Effect of Huangqi(Radix Astragali)-Gegen(Radix Pueraria Lobata)Compatibility on AMPK Signaling Pathway in Adipose Tissue of Diabetic Rats

作　　者：刘倩[1,2] 范颖[1,2] 李新[3] 刘丽[1,2] 李军[1,2] 林庶茹[1] LIU Qian;FAN Ying;LI Xin;LIU Li;LI Jun;LIN Shuru(Traditional Chinese Medicine School,Liaoning University of Traditional Chinese Medicine,Shenyang 110847,Liaoning,China;Key Laboratory of Traditional Chinese Medicine Theory and Applications of Education Ministry,Liaoning University of Traditional Chinese Medicine,Shenyang 110847,Liaoning,China;Information Engineering School,Liaoning University of Traditional Chinese Medicine,Shenyang 110847,Liaoning,China)

机构地区：[1]辽宁中医药大学中医学院,辽宁沈阳110847 [2]辽宁中医药大学中医脏象理论及应用教育部重点实验室,辽宁沈阳110847 [3]辽宁中医药大学信息工程学院,辽宁沈阳110847

出　　处：《中华中医药学刊》2021年第2期102-105,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(81273653)。

摘　　要：目的探讨黄芪与葛根配伍对糖尿病脂肪中腺苷酸活化蛋白激酶(AMPK)信号通路的作用,明确其调控血糖血脂机制。方法将86只SPF级SD雄性大鼠按照随机数字表分成6组:正常组、模型组、金芪降糖组、黄芪组、葛根组、黄芪葛根配伍组,正常组11只,其余各组15只大鼠。一次性腹腔注射2%STZ(46 mg/kg)造模,同日各组分别予金芪降糖混悬液1.47 g/kg、黄芪2.7 g/kg、葛根1.35 g/kg、黄芪葛根汤4.05 g/kg灌胃,给药30 d。酶联免疫吸附测定(ELISA)检测脂联素(APN)、葡萄糖转运体4(GLUT-4),实时荧光定量聚合酶链式反应法(Real-time PCR)检测脂联素受体1(AdipoR1)、脂联素受体2(AdipoR2)、AMPK、GLUT-4、过氧化物酶增殖体激活受体-α(PPARα)、PPARγmRNA。结果与正常组比较,模型组大鼠APN、GLUT-4水平降低,AdipoR1、AdipoR2、AMPK、GLUT-4、PPARα、PPARγmRNA表达减少(P<0.05)。黄芪、黄芪与葛根配伍能升高模型大鼠APN、GLUT-4水平(P<0.05);黄芪、葛根及两药配伍能增加AdipoR1(葛根、黄芪与葛根配伍除外)、AdipoR2、AMPK、PPARα(葛根除外)、PPARγ、GLUT-4mRNA表达(P<0.05),黄芪促进AdipoR2、PPARαmRNA表达的作用优于两药合用,黄芪与葛根配伍调节AMPK、PPARγ、GLUT-4mRNA表达的作用优于单一用药(P<0.05)。结论黄芪、葛根及其配伍调节血糖血脂与调控糖尿病大鼠脂肪组织AMPK信号通路有关,黄芪葛根配伍调控血糖、血脂作用优于单一用药,可能与其促进APN、AMPK、GLUT-4、PPARγmRNA表达有关。Objective To observe the effect of Huangqi(Radix Astragali)-Gegen(Radix Pueraria Lobata)compatibility on AMP-activated protein kinase(AMPK)signaling pathway in adipose tissue of diabetic rats and its mechanism of reducing blood glucose and blood lipid.Methods Eighty-six SPF male SD rats were divided into 6 groups:normal group,model group,Jinqi Jiangtang group,Huangqi(Radix Astragali)group,Gegen(Radix Pueraria Lobata)group and Huangqi(Radix Astragali)-Gegen(Radix Pueraria Lobata)compatibility group by the random number table.There were 11 rats in normal group and 15 rats in the other groups.Except the normal group,2%STZ(46 mg/kg)was given to rats of the other groups by intraperitoneal injection to induce diabetes models.At the same time,the suspension of Jinqi Jiangtang Tablet with the dose of 1.47 g/kg was given to rats in control group.Huangqi(Radix Astragali)with the dose of 2.7 g/kg was given to rats in Huangqi(Radix Astragali)group.Gegen(Radix Pueraria Lobata)with the dose of 1.35 g/kg was given to Gegen(Radix Pueraria Lobata)group.Huangqi Gegen Decoction with the dose of 4.05 g/kg were given to Huangqi(Radix Astragali)-Gegen(Radix Pueraria Lobata)compatibility group.All of drugs were given for 30 days continuously.The contents of adiponectin(APN)and glucose transporter4(GLUT-4)were detected by enzyme-linked immunosorbent assay(ELISA).The relative expression of adiponectin receptor 1(AdipoR1),adiponectin receptor 2(AdipoR2),AMPK,GLUT-4,peroxisome proliferator-activated receptorα(PPARα)and PPARγmRNA were tested by Real-time PCR.Results Compared with those of the normal group,the contents of APN and GLUT-4 were lowered,while the relative expressions of AdipoR1,AdipoR2,AMPK,GLUT-4,PPARαand PPARγmRNA were reduced in the model group(P<0.05).Huangqi(Radix Astragali)and Huangqi(Radix Astragali)-Gegen(Radix Pueraria Lobata)compatibility could increase APN and GLUT-4 in model group(P<0.05).Huangqi(Radix Astragali),Gegen(Radix Pueraria Lobata)and their compatibility could increase the relative expression of AdipoR

关 键 词：黄芪 葛根 中药配伍 糖尿病 脂肪组织 AMPK通路 

分 类 号：R285.5[医药卫生—中药学]