薰衣草精油对抑郁症大鼠海马、杏仁核pCREB及c-fos表达影响的研究  

Effects of Lavender Essential Oil on Expressions of pCREB and c-fos in Hippocampus and Amygdala of Depression Rats

作　　者：张轶[1] 贺芳[1] 何叶成[1] 宋悦宁[1] ZHANG Yi;HE Fang;HE Yecheng;SONG Yuening(Suzhou Vocational Health College,Suzhou 215009,Jiangsu,China)

机构地区：[1]苏州卫生职业技术学院,江苏苏州215009

出　　处：《中华中医药学刊》2021年第2期194-198,I0039,I0040,共7页Chinese Archives of Traditional Chinese Medicine

基　　金：江苏省卫生和计划生育委员会医学科研项目(J201504);苏州卫生职业技术学院科研项目(201710)。

摘　　要：目的探讨薰衣草精油对抑郁大鼠海马及杏仁核pCREB及c-fos的表达的影响及其对抑郁行为及学习记忆能力的改善作用。方法24只SD大鼠随机分为空白对照组(n=8)、抑郁对照组(n=8)、抑郁香薰组(n=8)。抑郁对照组和抑郁香薰组采用孤养加慢性不可预见性的温和应激(Chronic Unpredictable Mild Stress,CUMS)诱导抑郁症模型,造模后抑郁香薰组给持续吸入雾化薰衣草精油水溶液1 h,每天3次,持续28 d;抑郁对照组及空白对照组给吸入雾化的蒸馏水1 h,每天3次,持续28 d。采用旷场实验、糖水偏爱百分比及体质量及摄食量测定大鼠抑郁行为,采用Morris水迷宫测试记录大鼠学习记忆能力。采用免疫组化检测脑组织c-fos和pCREB的表达。结果(1)各组大鼠造模前糖水消耗百分比、水平运动及垂直活动得分差异无统计学意义(P>0.05)。造模后,抑郁对照组和抑郁香薰组大鼠糖水消耗百分比、水平活动得分和垂直活动得分均明显降低(P<0.05),各组大鼠造模前摄食量差异无统计学意义(P>0.05),造模后抑郁对照组及抑郁香薰组摄食量低于造模前(P<0.05),雾化后4周抑郁对照组摄食量同造模后比较差异无统计学意义(P>0.05),雾化后第3周及第4周抑郁香薰组摄食量高于造模后(P<0.05),但仍低于造模前(P<0.05)。雾化后第3周及第4周抑郁香薰组大鼠摄食量高于抑郁对照组(P<0.05)。各组造模前定位巡航潜伏期差异无统计学意义(P>0.05),造模后抑郁对照组及香薰对照组潜伏期延长(P<0.05),雾化吸入后4周抑郁香薰组级抑郁对照组潜伏期较造模后减少(P<0.05),但仍长于造模前(P<0.05),且抑郁对照组长于抑郁香薰组(P<0.05)。抑郁对照组及抑郁香薰组造模后第三象限路程百分比、第三象限时间百分比及穿越平台次数均减少(P<0.05),抑郁对照组及抑郁香薰组间差异无统计学意义(P>0.05),雾化吸入4周后抑郁对照组及抑郁香薰组第三象限路程�Objective To investigate the effect of lavender essential oil aromatherapy on expressions of pCREB and c-fos in hippocampus and improving learning and memory ability in depression rats.Methods Twenty-four SD rats were randomly divided into blank control group(n=8),depression control group(n=8)and depression aromatherapy group(n=8).Depression rat models were induced by separation and Chronic Unpredictable Mild Stress(CUMS).Rats in the depression aromatherapy group was given continuous inhalation of atomized lavender solution for 1 hour,three times a day,for 28 days and rats in blank control group and depression control group were given inhalation of atomized distilled water for 1 hour,three times a day,for 28 days,and depression rats were scored by open-field experiment,sugar preference percentage,weight and intake.Morris water maze test was used to record the learning and memory performance of rats.The immunohistochemical detection was used to test expressions of c-fos and pCREB in brain tissue.Results(1)There were no significant differences in the percentage of sugar water consumption,horizontal movement or vertical movement scores before modeling(P>0.05).After modeling,the consumption percentage of sugar and water,horizontal activity score and vertical activity score of depression control group and depression aromatherapy group were significantly reduced(P<0.05),and there was no significant difference in the food intake before modeling(P>0.05).The food intake of depression control group and depression aromatherapy group after modeling was lower than that before modeling(P<0.05),and there was no significant difference in the food intake of depression control group after four weeks of atomization compared with that after modeling(P>0.05).In the third and fourth week after atomization,the food intake of the depression group was higher than that of the model group(P<0.05),but it was still lower than that of the model group(P<0.05).At the 3 rd and 4 th week after atomization,the food intake of depression group was h

关 键 词：薰衣草精油 抑郁症模型大鼠 海马 杏仁核 pCREB及c-fos表达 

分 类 号：R285.5[医药卫生—中药学]