Influence of viscosity on chondrogenic differentiation of mesenchymal stem cells during 3D culture in viscous gelatin solution-embedded hydrogels  

作　　者：Kyubae Lee Yazhou Chen Xiaomeng Li Naoki Kawazoe Yingnan Yang Guoping Chen 

机构地区：[1]Research Center for Functional Materials,National Institute for Materials Science,1-1 Namiki,Tsukuba,Ibaraki,305-0044,Japan [2]Department of Materials Science and Engineering,Graduate School of Pure and Applied Sciences,University of Tsukuba,1-1-1 Tennodai,Tsukuba,Ibaraki,305-8577,Japan [3]School of Mechanics and Engineering Science,Zhengzhou University,Zhengzhou,450001,China [4]Graduate School of Life and Environmental Science,University of Tsukuba,1-1-1 Tennodai,Tsukuba,Ibaraki,305-8571,Japan

出　　处：《Journal of Materials Science & Technology》2021年第4期1-8,共8页材料科学技术（英文版）

基　　金：financially supported by JSPS KAKENHI(Nos.18K19947,18K19945 and 19H04475)。

摘　　要：Differentiation of human bone marrow-derived mesenchymal stem cells(hMSCs)is regulated by a variety of cues of their surrounding microenvironments.In particular,mechanical properties of cell culture matrices have been recently disclosed to play a pivotal role in stem cell differentiation.However,it remains elusive how viscosity affects the chondrogenic differentiation of hMSCs during three-dimensional(3 D)culture.In this study,a 3 D culture system that was established by embedding viscous gelatin solution in chemically cross-linked gelatin hydrogels was used for 3 D culture of hMSCs in gelatin solutions with different viscosities.The influence of solution viscosity on chondrogenic differentiation of hMSCs was investigated.Viscous gelatin solutions promoted cell proliferation in the order of low,middle and high viscosity while elastic hydrogels restricted cell proliferation.High viscosity gelatin solution led to increased production of the cartilaginous matrix.Under the synergistic stimulation of chondrogenic induction factors,high viscosity was beneficial for the chondrogenic differentiation of hMSCs.The results suggested the role of viscosity should be considered as one of the dominant mechanical cues affecting stem cell differentiation.

关 键 词：VISCOSITY CULTURE STIMULATION 

分 类 号：TQ427.26[化学工程] R318[医药卫生—生物医学工程]