The synergistic effect of 3D-printed microscale roughness surface and nanoscale feature on enhancing osteogenic differentiation and rapid osseointegration  

作　　者：Hui Wang Jiaqiang Liu Chengtao Wang Steve Guofang Shen Xudong Wang Kaili Lin 

机构地区：[1]Department of Oral and Cranio-maxillofacial Surgery,Shanghai Ninth People’s Hospital,College of Stomatology,Shanghai Jiao Tong University School of Medicine,National Clinical Research Center for Oral Diseases,Shanghai Key Laboratory of Stomatology&Shanghai Research Institute of Stomatology,Shanghai,200011,China [2]School&Hospital of Stomatology,Tongji University,Shanghai Engineering Research Center of Tooth Restoration and Regeneration,Shanghai,200072,China [3]School of Mechanical Engineering,Shanghai Jiao Tong University,Shanghai,200240,China [4]Shanghai University of Medicine&Health Sciences,Shanghai,201318,China

出　　处：《Journal of Materials Science & Technology》2021年第4期18-26,共9页材料科学技术（英文版）

基　　金：support by the Natural Science Foundation of China(81871490,81670958,81970973);Science and Technology Commission of Shanghai Municipality(17510710800,17410710500,19441902900);Program of Shanghai Academic/Technology Research Leader(19XD1434500);Pudong New Area Commission of Health and Family Planning(PW2016E1);Double Hundred Plan of Shanghai Jiao Tong University School of Medicine(20191819);Incentive Project of High-level Innovation Team for Shanghai Jiao Tong University School of Medicine。

摘　　要：Personalized precision therapy and rapid osseointegration are the main development directions of dental implants.3 D printing is a vital advanced manufacturing technology for personalized precision therapy.However,the osteogenesis of the 3 D printed Ti6 Al4 V implants is unsatisfactory.From the bionic perspective,the hierarchical micro/nano-topography can mimic the microenvironment of the multilevel structure of natural bone tissue and may endow the implant surface with superior bioactivity.In the present study,the hierarchical micro/nano-topography was successfully fabricated by construction the nanoscale feature on 3 D printed microscale roughness surface of 3 D-printed Ti6 Al4 V implants by alkali-heat treatment and hydrothermal treatment.Then the cell biological responses in vitro and osseointegration performance in vivo were systematically evaluated.The hierarchical micro/nano-topography evidently increased the roughness,improved the hydrophilicity and accelerated the hydroxyapatite deposition and mineralization,which significantly enhanced the adhesion,differentiation and extracellular matrix mineralization of bone marrow derived mesenchymal stromal cells(BMSCs).Most importantly,the hierarchical micro/nano-topography on 3 D-printed implants facilitated the new bone formation and rapid osseointegration in vivo.Our study suggested that 3 D-printed implant with micro/nano-topography may be a promising candidate to be applied in orthopedic field to meet the need of customized therapy and rapid osseointegration.

关 键 词：RAPID ROUGHNESS TOPOGRAPHY 

分 类 号：R318[医药卫生—生物医学工程]