Silk microfibrous mats with long-lasting antimicrobial function  

作　　者：Mi Wu Wen Liu Jinrong Yao Zhengzhong Shao Xin Chen 

机构地区：[1]State Key Laboratory of Molecular Engineering of Polymers,Department of Macromolecular Science,Laboratory of Advanced Materials,Fudan University,Shanghai 200433,PR China

出　　处：《Journal of Materials Science & Technology》2021年第4期203-209,共7页材料科学技术（英文版）

基　　金：supported by the National Natural Science Foundation of China(No.21574023,21574024,and 21935002)。

摘　　要：The controlled release of antibiotic drugs to injured sites has great advantages over the conventional intravenous administration of antibiotics,which is associated with systemic toxicity,for wound care.Electrospun nanofibrous/microfibrous mats,with a similar structure to the native extracellular matrix,is a promising wound dressing.Herein,drug-loaded halloysite nanotubes(HNTs)incorporated into regenerated silk fibroin(RSF)microfibrous mats were prepared by electrospinning to achieve sustained drug release and long-lasting antimicrobial protection.A broad-spectrum antibiotic,tetracycline hydrochloride(TCH),was selected as the model drug.Transmission electron microscopic images revealed that the TCH-loaded HNTs were homogeneously embedded in the RSF electrospun microfibers without significant changes in morphology.The drug release profiles showed that the RSF microfibrous mats with TCH-loaded HNTs exhibited a significantly reduced burst phase and a long release time over two weeks compared to the pure TCH-loaded HNTs and the TCH-loaded RSF microfibrous mats without HNTs.These results were attributed to the two-step release of TCH first from the HNTs and then RSF matrix in the electrospun mats.Finally,the antimicrobial properties of the RSF microfibrous mats with TCH-loaded HNTs were evaluated using both Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli bacteria.The results demonstrated long-lasting antimicrobial activity for at least one week,showing the great potential of TCH-loaded RSF microfibrous mat as a wound dressing material.Therefore,these TCHloaded RSF microfibrous mats with excellent biocompatibility and sustained antimicrobial protection are extremely attractive systems for clinical applications.

关 键 词：loaded RELEASE SUSTAINED 

分 类 号：R318[医药卫生—生物医学工程] TQ342[医药卫生—基础医学]