沉默乳腺癌扩增序列2基因对乳腺癌MDA-MB-231细胞凋亡的影响  

Effect of silencing breast cancer amplified sequence 2 gene on apoptosis in MDA-MB-231 cells

作　　者：韩迎龙 李清漪[1] 韩翠翠[1] 都晓辉[1] 丛欢[1] 包亚男[1] 林宇[1] HAN Ying-long;LI Qing-yi;HAN Cui-cui;DOU Xiao-hui;CONG Huan;BAO Ya-nan;LIN Yu(School of Pharmacy,Qiqihar Medical University,Qiqihar 161006,Heilongjiang Province,China)

机构地区：[1]齐齐哈尔医学院药学院,黑龙江齐齐哈尔161006

出　　处：《中国临床药理学杂志》2021年第5期519-521,共3页The Chinese Journal of Clinical Pharmacology

基　　金：齐齐哈尔医学院博士基金资助项目(QY2016B-34)。

摘　　要：目的研究沉默乳腺癌扩增序列2(BCAS2)基因对乳腺癌MDA-MB-231细胞凋亡的影响。方法将细胞分3组:空白组、对照组和实验组。空白组细胞不做任何处理,对照组细胞转染空载体(3×10^(8)TU·mL^(-1),10μL),实验组细胞转染慢病毒载体pGLV3-GFP-shBCAS2(3×10^(8)TU·mL^(-1),10μL)。用AnnexinV-FITC/PI双染法检测细胞凋亡率;用试剂盒法检检测Caspase-3活性(光密度值);蛋白质印迹法检测caspase-3蛋白表达(灰度值)。结果空白组、对照组和实验组的细胞凋亡率分别为(12.33±1.08)%,(12.56±0.77)%和(44.93±3.22)%;这3组的Caspase-3活性分别为1.00±0.06,1.02±0.07和3.91±0.70;这3组的caspase-3蛋白相对表达量分别为0.21±0.02,0.19±0.03和0.42±0.04。实验组与空白组和对照组相比,上述指标的差异均有统计学意义(均P<0.01)。结论沉默BCAS2基因可诱导乳腺癌MDA-MB-231细胞凋亡,其机制可能与caspase-3激活有关。Objective To investigate the effect of breast cancer amplified sequence 2(BCAS2)silence on apoptosis in breast cancer MDA-MB-231 cells.Methods Cells were divided into three groups:blank group,control group and experimental group.The blank group was transfected nothing,the control group was transfected with control vector(3×10^(8)TU·mL^(-1),10μL),while the experimental group was transfected with lentivirus vector shBCAS2(3×10^(8)TU·mL^(-1),10μL).The cell apoptosis was detected by Annexinv-FITC/PI double staining.The activity(optical density)of caspase-3 was assessed by detection kit.The protein level(gray value)of caspase-3 was detected by Western blot.Results The apoptosis rates in the blank,control and experimental groups were(12.33±1.08)%,(12.56±0.77)%and(44.93±3.22)%,respectively;the activity of capase-3 in the three groups were 1.00±0.06,1.02±0.07 and 3.91±0.70,respectively;the protein expressions of caspase-3 in the three groups were 0.21±0.02,0.19±0.03 and 0.42±0.04.Comparison between experimental group and blank or control group,the difference of the factors were significant(all P<0.01).Conclusion Silencing of BCAS2 can promote apoptosis of breast cancer MDA-MB-231 cells,which might be related to activation of caspase-3.

关 键 词：乳腺癌扩增序列2 乳腺癌 MDA-MB-231细胞 凋亡 半胱氨酸蛋白酶3 

分 类 号：R979.1[医药卫生—药品]