微波治疗术联合派特灵对尖锐湿疣患者外周血自然杀伤T细胞及相关细胞因子影响  

Effect of microwave therapy combined with Patelin on natural killer T cells and related cytokines in peripheral blood of patients with condyloma acuminatum

作　　者：马军格[1] 李辉霞 杨长乐 侯冰 程瑞玲[1] 玉美花[1] 李立红[1] MA Junge;LI Huixia;YANG Changle;HOU Bing;CHEN Ruiling;YU Meihua;LI Lihong(Department of Dermatology,Handan Central Hospital,Handan Hebei,056001,China;Department of Obstetrics and Gynecology,Handan Seventh Hospital,Handan Hebei,056001,China)

机构地区：[1]邯郸市中心医院皮肤科,河北邯郸056001 [2]邯郸市第七医院妇产科,河北邯郸056001

出　　处：《新疆医科大学学报》2021年第3期339-343,共5页Journal of Xinjiang Medical University

基　　金：河北省中医药管理局科研计划项目(2020603)。

摘　　要：目的探讨微波治疗术联合派特灵对尖锐湿疣患者外周血自然杀伤T细胞及相关细胞因子的影响。方法共128例尖锐湿疣患者,均为2018年1月-2019年6月期间在本院治疗患者,按照随机数表法分为对照组和研究组,各64例,对照组患者采用微波治疗术治疗,研究组在对照组基础上使用派特灵治疗,观察记录两组患者治疗后3、6、9个月复发例数,并计算复发率,使用流式细胞仪检测患者细胞免疫学指标CD8^(+)、CD4^(+)、CD3^(+)、自然杀伤T细胞(natural killer T cell,NTK)水平,并计算CD4^(+)/CD8^(+)值,其中以NTK占淋巴细胞数目的比值进行分析,观察治疗前后患者CD8^(+)T细胞、CD4^(+)T细胞内白细胞介素-2(Interleukin-2,IL-2)、白细胞介素-4(Interleukin-4,IL-4)、白细胞介素-12(Interleukin-12,IL-12)及γ干扰素(IFN-γ)染色阳性细胞百分率,记录两组治疗效果。结果研究组患者治疗3个月后复发率为1.56%,治疗6个月后复发率为6.25%,治疗9个月后复发率为7.81%,低于对照组的14.06%、23.44%和35.94%,差异有统计学意义(P<0.05);治疗前,两组患者CD4^(+)/CD8^(+)、CD8^(+)、CD4^(+)、CD3^(+)和NTK差异无统计学意义(P>0.05),治疗后,两组患者CD4^(+)/CD8^(+)、CD8^(+)、CD4^(+)、CD3^(+)和NTK均升高,且研究组患者CD4^(+)/CD8^(+)、CD8^(+)、CD4^(+)、CD3^(+)和NTK均高于对照组,差异有统计学意义(P<0.05);治疗前,两组患者CD8^(+)T细胞、CD4^(+)T细胞中细胞因子IFN-γ、IL-12、IL-4和IL-2染色阳性细胞百分率差异无统计学意义(P>0.05),经过治疗,两组患者CD8^(+)T细胞、CD4^(+)T细胞中细胞因子IFN-γ、IL-12、IL-4和IL-2染色阳性细胞百分率均升高,且研究组患者均大于对照组,差异有统计学意义(P<0.05);研究组患者总有效率96.87%,高于对照组的79.69%,差异有统计学意义(P<0.05)。结论微波治疗术联合派特灵可降低尖锐湿疣患者复发率,提高患者外周血自然杀伤T细胞及相关细胞因子水平,增强免疫能力,Objective To explore the effect of microwave therapy combined with Patelin on natural killer T cells and related cytokines in patients with condyloma acuminatum.Methods A total of 128 patients with condyloma acuminatum were treated in this study,all of whom were treated in our hospital from January 2018 to June 2019.They were divided into two groups randomly:control group and study group,with 64 patients for each.The control group was treated with microwave therapy,while the study group was treated with Patelin on the basis of the control group.The number of relapses at 3,6,and 9 months after treatment was observed and recorded,and the recurrence rate was calculated.CD8^(+),CD4^(+),CD3^(+),and natural killer T cell(NTK)levels were detected by flow cytometry,and CD4^(+)/CD8^(+)and the ratio of NTK to lymphocyte number were calculated and analyzed.The percentage of IL-2,IL-4,IL-12,and IFN-γpositive cells in CD8^(+)T cells and CD4^(+)T cells of patients before and after treatment were determined,and the treatment effect of the two groups was recorded.Results The relapse rate of patients in the study group was 1.56%,6.25%and 7.81%after 3,6 and 9 months of the treatment respectively,while that in the control group was 14.6%,23.44%and 35.94%respectively(P<0.05);before the treatment,there were no significant differences in CD4^(+)/CD8^(+),CD8^(+),CD4^(+),CD3^(+),and NTK between the two groups(P>0.05).After the treatment,CD4^(+)/CD8^(+),CD8^(+),CD4^(+),CD3^(+)and NTK of the two groups were increased,and they were all higher in the study group than those in the control group(P<0.05).Before the treatment,there was no significant difference in the percentage of IL-2,IL-4,IL-12 and IFN-γstaining positive cells in CD8^(+)T cells,CD4^(+)T cell of the two groups of patients(P>0.05).After the treatment,the percentage of IL-2,IL-4,IL-12 and IFN-γstaining positive cells in CD8^(+)T cells and CD4^(+)T cells in the two groups were higher than those in the control group(P<0.05);the total effective rate of patients in the group was

关 键 词：派特灵 尖锐湿疣 微波治疗术 治疗效果 细胞因子 

分 类 号：R692.5[医药卫生—泌尿科学]