利用SSR分子标记技术快速鉴定津甜100甜瓜种子纯度  

Rapid identification of seed purity of‘Jintian 100’oriental melon by SSR

作　　者：武云鹏 李肯 张若纬[1] 彭冬秀[1] WU Yunpeng;LI Ken;ZHANG Ruowei;PENG Dongxiu(State Key Laboratory of Vegetable Gemplasm Innovation/Tianjin Enterprise Key Laboratory of Vegetable Genetics&Breeding/Vegetable Research Institute,Tianjin Kernel Agricultural Science&Technology Corporation Limited,Tianjin 300381,China)

机构地区：[1]蔬菜种质创新国家重点实验室·天津市蔬菜遗传育种企业重点实验室·天津科润农业科技股份有限公司蔬菜研究所,天津300381

出　　处：《中国瓜菜》2021年第3期27-30,共4页China Cucurbits And Vegetables

基　　金：天津市科技计划项目(17ZXBFNC00200)。

摘　　要：利用SSR分子标记技术鉴定甜瓜种子纯度,是甜瓜种子生产、销售过程中的关键环节,对甜瓜产业发展具有重要意义。利用津甜100亲本对24对甜瓜SSR引物进行多态性筛选,得到多态性高、在F_(1)代中条带差异较大的2对引物。利用聚丙烯酰胺凝胶电泳技术对F_(1)群体进行鉴定,鉴定结果与田间鉴定结果一致,纯度均为99.0%。因此,笔者筛选得到多态性高、条带差异大的2对引物,可鉴定津甜100甜瓜种子。Identification of the melon seeds purity by SSR molecular marker technology is the key link in the process of melon seeds production and sales.I24 pairs of SSR primers of oriental melon were used to screen for the polymorphism of Jintan 100 parents,2 pairs of primers with high polymorphic in F_(1) generation were obtained.The purity of F_(1) population was identified by polyacrylamide gel electrophoresis using the 2 pairs,the molecular identification results were consistent with the field identification results,and the purity was 99%.Therefore,two pairs of primers with high polymorphism were screened to identify Jintian 100 oriental melon seeds.

关 键 词：甜瓜 分子标记 引物 快速检测 

分 类 号：S652[农业科学—果树学]