2014-2016年镇江市甲型H3N2流感病毒HA和NA基因遗传进化研究  

Genetic evolutional analysis of the hemagglutinin and neuraminidase genes of influenza A H3N2 viruses in Zhenjiang city from 2014 to 2016

作　　者：杨静[1] 沈俐[1] 刘金[1] 张潇丹[1] 吴霄[1] 徐虹[1] YANG Jing;SHEN Li;LIU Jin;ZHANG Xiao-dan;WU Xiao;XU Hong(Zhenjiang Municipal Center for Disease Control and Prevention,Jiangsu Zhenjiang 212003,China)

机构地区：[1]镇江市疾病预防控制中心,江苏镇江212003

出　　处：《江苏预防医学》2021年第1期8-12,共5页Jiangsu Journal of Preventive Medicine

基　　金：镇江市科技项目(SH2018076,SH2019026)。

摘　　要：目的对2014-2016年镇江市甲型H3N2流感病毒的HA和NA基因进行分子流行病学分析,了解其进化特征。方法收集2014-2016年镇江市流感样病例咽拭子标本,对核酸检测鉴定为阳性的标本进行病毒分离;随机抽取13株经血凝抑制试验(HI)鉴定确认为甲型H3N2亚型毒株,用特异性的引物扩增其HA和NA基因,进行序列分析。对分离株和疫苗株、参比毒株的核苷酸、氨基酸同源性进行分析,对其抗原表位、耐药位点和糖基化位点进行分析。结果 2014-2016年镇江市共采集9 532份流感样标本,流感病毒阳性检出率为11.77%,3年分别为11.23%、13.82%和10.61%,差异有统计学意义(χ^(2)=16.602,P<0.001)。甲型H3N2亚型为最主要的亚型(占43.76%),其次为乙型BY型(占35.65%);除2015年乙型BY亚型为主(占70.13%),2014年、2016年均以甲型H3N2亚型为主(分别占48.30%、54.80%)。与北半球疫苗株A/Texas/50/2012(2013-2015)及A/Switzerland/9715293/2013(2015-2016)比对,选取的13株甲型H3N2镇江分离株的HA和NA基因核苷酸、氨基酸的同源性均≥97.4%,与北半球疫苗株HA和NA基因核苷酸、氨基酸的同源性均≥94.8%。13株镇江分离H3N2毒株主要分为4个进化分支。13株均未出现主要抗原位点氨基酸序列缺失,存在Y94H、A128T、S138A、G142R等散点性突变。与M2、NA蛋白相关的耐药位点未发现变异;3株糖基化位点发生D329N氨基酸替换。结论甲型H3N2亚型流感病毒是镇江市优势流行亚型。主要抗原位点、耐药位点均未发现重要突变,糖基化位点相对保守,仍应继续加强监测。Objective To analyze the genetic characteristics of the hemagglutinin(HA)and neuraminidase(NA)genes of influenza A H3 N2 virus in Zhenjiang city from 2014 to 2016;to understand its evolutionary characteristics.Methods The throat swab samples of influenza like(ILI)cases in Zhenjiang city from 2014 to 2016 were collected,and the virus was isolated from positive samples detected by nucleic acid detection;13 H3 N2 subtype A virus strains were randomly selected and subjected to hemagglutination inhibition(HI)test,and their HA and NA genes were amplified with specific primers for sequence analysis.The nucleotide and amino acid homology of isolates,vaccine strains and reference strains were calculated,and the antigenic epitopes,drug resistance sites and glycosylation sites were analyzed.Results A total of 9 532 ILI samples were collected in Zhenjiang city from 2014 to 2016.The positive detection rate of influenza virus was 11.77%,and the 3-year positive detection rates were 11.23%,13.82% and 10.61%,respectively,with significant difference (χ2=16.602,P <0.001).Influenza A H3 N2 subtype was dominant prevalence strains,followed by BY subtype(accounted for 35.65%).Except for2015,BY subtype was the dominant strain(accounted for 70.13%);A H3 N2 subtype was the dominant subtype in 2014 and2016(accounted for 48.30%and 54.80%,respectively).The nucleotide and amino acid homologies of HA and NA genes of 13 strains of A H3 N2 isolates were above 97.4%.Compared with the northern hemisphere vaccine strain A/Texas/50/2012(2013-2015)and A/Switzerland/9715293/2013(2015-2016),the nucleotide and amino acid homologies of HA and NA genes were above 94.8%.The 13 H3 N2 strains were mainly divided into 4 evolutionary branches.None of the 13 strains showed deletion of amino acid sequence of major antigen sites,and there were scattered mutations such as Y94 H,A128 T,S138 Aand G142 R.No mutation was found in the resistance sites associated with M2 and NA proteins,and D329 Namino acid substitution was found in the glycosylation sites of 3 strains

关 键 词：甲型H3N2流感病毒 HA基因 NA基因 遗传进化 

分 类 号：R373.1[医药卫生—病原生物学]