金银花药材、标准汤剂、中间体、配方颗粒HPLC特征图谱相关性研究  

Study on correlation of characteristic chromatograms of pieces,standard decoction,intermediate and formula granule of Lonicera japonica flos by HPLC

作　　者：郭晨 李夕 郭珮怡 杨建刚 杨海燕 曹爱兰 张俏 GUO Chen;LI Xi;GUO Peiyi;YANG Jiangang;YANG Haiyan;CAO Ailan;ZHANG Qiao(Drug Quality Research Division,Medicine Researchinstitute of Shaanxi Pharmaceutical Holding Cooperation,Shaanxi Province,Xi’an710000,China;Shaanxi Institute for Food and Drug Control,Shaanxi Province,Xi’an710000,China;Shaanxi Chinese Medicine InstituteShaanxi Medical Information Center,Shaanxi Province,Xianyang712000,China)

机构地区：[1]陕西医药控股医药研究院有限公司药物质量研究部,陕西西安710000 [2]陕西省食品药品监督检验研究院,陕西西安710000 [3]陕西中药研究所陕西医药信息中心,陕西咸阳712000

出　　处：《中国医药导报》2021年第7期34-38,共5页China Medical Herald

基　　金：陕西省创新能力支撑计划项目(2018PT-29);陕西省技术创新引导专项(基金)项目(2018HJCG-09)。

摘　　要：目的研究金银花药材、标准汤剂、中间体、配方颗粒特征图谱的相关性。方法采用HPLC法进行研究,使用Thermo Scientific AcclaimTM120 C18色谱柱(5μm,4.6×250 mm);以乙腈-0.4%磷酸水溶液为流动相进行梯度洗脱;检测波长为327 nm;柱温为30℃;流速为1 mL/min;进样量为10μL。比较15批金银花药材、标准汤剂、中间体、配方颗粒特征图谱及相关性。结果金银花药材、标准汤剂、中间体、配方颗粒特征图谱均可指认出新绿原酸、绿原酸、隐绿原酸、异绿原酸B、异绿原酸A、异绿原酸C等6个成分。15批金银花药材、标准汤剂、中间体、配方颗粒各特征图谱相似度均大于0.9。金银花药材、标准汤剂、中间体、配方颗粒中主要化学成分组成基本相同。结论建立的HPLC法特征图谱方法可用于金银花药材、标准汤剂、中间体、配方颗粒的质量控制。Objective To study the correlation of characteristic chromatograms of pieces,standard decoction,intermediate and formula granule of Lonicera japonica flos.Methods The results were studied by HPLC method;column was Thermo Scientific AcclaimTM120 C18(5μm,4.6×250 mm);acetonitrile-0.4%phosphoric acid aqueous solution was used as mobile phase for gradient elution.The detection wavelength was 327 nm.The column temperature was 30℃.The flow rate was 1 mL/min.The injection volume was 10μL.Characteristic chromatograms and correlation with 15 batches of pieces,standard decoction,intermediate and formula granule of Lonicera japonica flos were compared.Results Six components,including neochlorogenic acid,chlorogenic acid,crypto-chlorogenic acid,isochlorogenic acid B,isochlorogenic acid A and isochlorogenic acid C,were identified by the characteristic chromatograms for 15 batches of pieces,standard decoction,intermediate and formula granule of Lonicera japonica flos.The similarity of characteristic chromatograms of 15 batches of pieces,standard decoction,intermediate and formula granule of Lonicera japonica flos were all greater than 0.9.The main chemical components of pieces,standard decoction,intermediate and formula granule of Lonicera japonica flos were basically the same.Conclusion The established HPLC characteristic chromatograms method can be used for the quality control of pieces,standard decoction,intermediate and formula granule of Lonicera japonica flos.

关 键 词：金银花药材 标准汤剂 中间体 配方颗粒 特征图谱 绿原酸 

分 类 号：R284.1[医药卫生—中药学]