根际细菌吡咯伯克霍尔德氏菌YZU-S377对棉花黄萎病的防效及其促生作用研究  

Control Effect of Burkholderia pyrrocinia YZU-S377 on Cotton Verticillium Wilt and Its Growth Promotion Function

作　　者：刘璐 万伟杰 郑通文 龙欣钰 孙正祥[1] 周燚[1] LIU Lu;WAN Weijie;ZHENG Tongwen;LONG Xinyu;SUN Zhengxiang;ZHOU Yi(Agricultural College of Yangtze University/Hubei Provincial Agricultural and Forestry Pest and Disease Early Warning and Regulation Engineering Technology Research Center,Jingzhou 434025,China)

机构地区：[1]长江大学农学院/湖北省农林病虫害预警与调控工程技术研究中心,湖北荆州434025

出　　处：《河南农业科学》2021年第3期91-96,共6页Journal of Henan Agricultural Sciences

基　　金：湖北省教育厅重点项目(D20181304)。

摘　　要：吡咯伯克霍尔德氏菌(Burkholderia pyrrocinia)YZU-S377(S377)是前期获得的1株水稻根际细菌,对植物病菌具有广谱抑制作用。为探究S377对棉花黄萎病的生防潜力,测定了菌株S377对棉花黄萎病的盆栽防效、在棉花植株内的定殖动态及其促生作用。结果表明,菌株S377对棉花黄萎病菌(Verticillium dahliae)的相对抑制率为89.22%,盆栽防效为62.53%;经S377无菌滤液处理后,棉花种子发芽率提高了22.22个百分点,棉花幼苗根长增加了27.78 mm,地上鲜质量增加了0.73 g;菌株S377可以形成生物被膜,在OD 600等于0.15和0.25时形成生物被膜强度较显著,在棉花植株根部可以持续定殖21 d,其数量呈先增后减趋势。上述结果表明,菌株S377对棉花黄萎病具有良好的生防潜力。Burkholderia pyrrocinia YZU-S377(S377)is a rice rhizosphere bacterium obtained in the early stage,which has broad-spectrum inhibitory effect on plant pathogens.To verify the biocontrol potential of S377 against Verticillium dahliae,the control effect of S377 on cotton Verticillium wilt,the colonization dynamics and growth promoting effect of S377 on cotton were tested in pot.The results showed that the relative inhibition rate of the strain to V.dahliae was 89.22%,and the control effect of pot test was 62.53%.After treated with S377 sterile filtrate the germination rate of cotton increased by 22.22 percentage points,the root length of cotton seedlings increased by 27.78 mm,and the fresh weight of aboveground part increased by 0.73 g.The results of crystal violet staining showed that strain S377 had the ability to form biofilm.The biofilm formation intensity of strain S377 at OD 600=0.15 and 0.25 was significantly more obvious than that under other concentrations.The colonization experiment showed that S377 could colonize cotton roots for 21 days,and the number increased first and then decreased.The results indicated that S377 was a potential biocontrol strain against cotton Verticillium wilt.

关 键 词：吡咯伯克霍尔德氏菌 棉花黄萎病菌 生物防治 盆栽防效 促生作用 定殖动态 

分 类 号：S436.611.1[农业科学—农业昆虫与害虫防治]