贵州猕猴桃果实腐烂病病原菌鉴定及分析  

Identification and Analysis of Pathogen of Kiwifruit Rot in Guizhou Province

作　　者：苏文文 吴迪 韩振诚 李良良 李苇洁 任春光 SU Wenwen;WU Di;HAN Zhencheng;LI Liangliang;LI Weijie;REN Chunguang(Guizhou Mountain Resources Research Institute,Guiyang 550001,China)

机构地区：[1]贵州省山地资源研究所,贵州贵阳550001

出　　处：《河南农业科学》2021年第3期97-102,共6页Journal of Henan Agricultural Sciences

基　　金：贵州省科技支撑计划项目(黔科合支撑[2017]2569);贵州省科技计划项目(黔科合基础[2016]1140号,黔科合基础[2017]1174号);贵州省科研机构创新能力建设项目(黔科合服企[2019]4004号);贵州科学院青年基金项目(黔科院J合字[2017]17号,黔科院J合字[2019]10号)。

摘　　要：为明确贵州省猕猴桃主栽品种果实腐烂病病原菌的种类,并为田间果实病害防治提供理论依据,以2018—2019年在贵州省水城县和修文县两大主产区采集的红阳和贵长猕猴桃病果为试验材料,通过病原菌的分离、形态学观察、分子检测和致病性检测,研究病果中病原真菌的种类,并对其序列进行系统发育分析。结果表明,分离到4类病原菌:拟茎点霉菌(Phomopsis cauloides)、镰刀菌(Fusarium)、交链格孢菌(Alternaria alternata)、黄瓜织球壳菌(Plectosphaerella cucumerina),其检出率分别为10%、60%、20%、10%。其中,黄瓜织球壳菌侵染引起猕猴桃果实病害在国内尚属首次报道。In order to clarify the types of the pathogens of fruit rot disease of main kiwifruit varieties in Guizhou Province,and provide theoretical basis for the prevention and control of field fruit diseases,the diseased fruits of Hongyang and Guichang kiwifruit collected in Shuicheng County and Xiuwen County of Guizhou Province from 2018 to 2019 were used as experimental materials.The pathogens were identified through pathogen isolation,morphological observation,molecular detection and pathogenicity detection.The phylogenetic analysis of pathogen species based on their sequences were carried out.The results showed that four kinds of pathogens including Phomopsis cauloides,Fusarium,Alternaria alternata,Plectosphaerella cucumerina were isolated,and their detection rates were 10%,60%,20%,10%respectively.The fruit disease of kiwifruit caused by the infection of Plectosphaerella cucumerina was reported for the first time in China.

关 键 词：猕猴桃 腐烂病 病原菌鉴定 序列分析 致病性检测 拟茎点霉菌 镰刀菌 黄瓜织球壳菌 

分 类 号：S432.1[农业科学—植物病理学]