荔枝核总黄酮联合紫杉醇对前列腺癌耐药细胞的增殖抑制作用  

The anti-proliferation effect of total flavonoids of litchi seed combined with paclitaxel on prostate cancer paclitaxel resistant cells

作　　者：张维权 李小兰 薛薇[1,3] 常明 郭宏伟[1,2] ZHANG Wei-quan;LI Xiao-lan;XUE Wei;CHANG Ming;GUO Hong-wei(College of Pharmacy,Guangxi Medical University;Key Lab of Longevity and Aging-Related Diseases of Chinese Ministry of Education&Center for Translational Medicine,Nanning 530021,China;Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine,Nanning 530011,China)

机构地区：[1]广西医科大学药学院,广西南宁530021 [2]长寿与老年相关疾病教育部重点实验室&广西医科大学转化医学研究中心,广西南宁530021 [3]广西中医药大学附属瑞康医院,广西南宁530011

出　　处：《中国药理学通报》2021年第4期528-534,共7页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81660681);广西一流学科建设项目(药学)(No 02401218004C)。

摘　　要：目的探讨荔枝核总黄酮(Total flavonoids of litchi seed,TFLS)是否增强前列腺癌紫杉醇耐药细胞(Prostate cancer paclitaxel resistance cells,PCa-Txr)对紫杉醇(Paclitaxel,PTX)的敏感性,以及TFLS与PTX联合应用是否具有协同抑制作用。方法采用CCK-8法检测TFLS和PTX对PCa-Txr细胞及亲本细胞的增殖抑制作用;采用低细胞毒性剂量TFLS提前处理或与PTX同时作用PCa-Txr细胞来观察细胞对PTX敏感性;采用Chou T C推荐的联合方案来观察TFLS与PTX联合应用对PCa-Txr细胞的增殖抑制作用,并依据联合指数(Combination index,CI)判断TFLS与PTX联合应用对PCa-Txr细胞是否具有协同抑制作用。结果TFLS抑制PCa-Txr细胞及亲本细胞的增殖,并呈现时间和剂量依赖性。TFLS提前处理或与PTX同时作用PCa-Txr细胞均未能增加耐药细胞对PTX的敏感性。TFLS联合PTX明显抑制PCa-Txr细胞活性,CI值小于1,两药联合应用存在协同作用。结论TFLS抑制PCa-Txr细胞的增殖,TFLS联合PTX协同地抑制PCa-Txr细胞,但TFLS未能增加PCa-Txr细胞对PTX的敏感性。Aim To investigate the sensitization effect of total flavonoids of litchi seed(TFLS)to paclitaxel(PTX)on prostate cancer paclitaxel resistance(PCa-Txr)cells and the synergistic inhibitory effect of TFLS combined with PTX.Methods CCK-8 method was carried out to detect the inhibitory effect of TFLS and PTX on PCa-Txr cells as well as corresponding parental cells.PCa-Txr cells were either pre-treated or simultaneously treated with low cytotoxic dose of TFLS to observe the sensitivity of PCa-Txr cells to PTX.The synergistic inhibitory effect of TFLS combined with PTX on the proliferation of PCa-Txr cells were observed according to the method recommended by Chou T C.Combination index(CI)was used to determine whether TFLS and PTX had synergistic inhibitory effect.Results TFLS significantly inhibited the proliferation of PCa-Txr cells and parental cells in a certain time-and dose-dependent manner.The low dose of TFLS pre-treated or treated with PTX simultaneously failed to increase the sensitivity of PTX on PCa-Txr cells.TFLS combined with PTX significantly inhibited the proliferation of PCa-Txr cells with CI value less than one.Conclusions TFLS inhibits the proliferation of PCa-Txr cells,while TFLS combined with PTX has synergistic inhibitory effect on PCa-Txr cells.However,TFLS fails to increase the sensitivity of PCa-Txr cells to PTX.

关 键 词：荔枝核总黄酮 紫杉醇 前列腺癌 耐药细胞 增敏 协同作用 

分 类 号：R282.71[医药卫生—中药学] R285.5[医药卫生—中医学]